biostudies-arrayexpressKanishk AsthanaHomo sapiensChronoSeq-Toolshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15894Both Live-cell imaging studies and scRNA-seq studies have shown multiple heterogeneous responses to the same stimulus in the same cells. Therefore as a first application we investigated if ChronoSeq could also reveal the heterogeneity in stimulus response; we applied ChronoSeq to profile inflammatory responses in K562 cells stimulated with TNF-α, to capture the rapid NF-κB signaling cascade. 10 minute interval between samples including a 1 minute sampling duration.biostudies-arrayexpressLibrary Construction - ChronoSeq library preparation protocol is very similar to Drop-seq. Beads from all time-tags are pooled together, then split into four equal parts for Reverse Transcription followed by PCR amplification.Sequencing - Illumina NovaSeq X 10B on one lane in PE100 configuration with Custom Read 1 primerSample Collection - Automated cell sampling using ChronoSeq device. Each cell sample is injected with a unique injection of Time-Tagged ChronoSeq beads.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - hg38 alignment. Data processed using ChronoSeq-Tools pipeline.Data Transformation - UMIs and gene information were used to generate unique transcripts captured and remove PCR duplicates using Drop-seq tools.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XChronoSeqRNA-seq of coding RNA from single cellsHomo sapiensKanishk AsthanafalseK562 cells treated with 10ng/ml TNFα, profiled with ChronoSeq for 12 samples barcoded with unique Time-TagsBoth Live-cell imaging studies and scRNA-seq studies have shown multiple heterogeneous responses to the same stimulus in the same cells. Therefore as a first application we investigated if ChronoSeq could also reveal the heterogeneity in stimulus response; we applied ChronoSeq to profile inflammatory responses in K562 cells stimulated with TNF-α, to capture the rapid NF-κB signaling cascade. 10 minute interval between samples including a 1 minute sampling duration.2025-11-01T00:00:00Z2026-05-26T13:06:43.947Z2025-10-29T06:15:13.916ZE-MTAB-15894ERP183239EFO_0004170EFO_0005684EFO_0004917EFO_0005518EFO_0003816EFO_0004184