{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Rajgopal Govindarajan"],"study_type":["transcription profiling by array"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15905"],"description":["Despite the well-established role of equilibrative nucleoside transporters (ENTs) in salvaging nucleosides for DNA synthesis, the presence of multiple ENT subfamilies within a single genome suggests putative, non-redundant functions in maintaining cellular homeostasis. In this study, we demonstrate that, in contrast to endolysosomal ENT3, which promotes autophagy, cell surface-localized ENT1 is capable of inhibiting autophagy by suppressing AMPK phosphorylation. Consistent with this, silencing ENT1 induces autophagy, whereas silencing ENT3 suppresses it.  ENT1⁻/⁻ and ENT3⁻/⁻ mice display autophagic proficiency and deficiency, respectively. These findings underscore a dynamic and reciprocal regulatory relationship between ENT1 and ENT3 in autophagy, offering new avenues for therapeutic modulation in autophagy-related disorders."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Hybridization - The labeled cRNA (750 ng) was then hybridized to Illumina MouseRef-8 v2.0 Expression BeadChips for 16 hours at 58°C with constant agitation. Following hybridization, chips were washed and stained with streptavidin using standard Illumina protocols.","Labeling - 100-500 ng of total RNA for each sample was used to synthesize biotin-labeled complementary RNA (cRNA) using the Illumina TotalPrep RNA Amplification Kit (#AMIL1791, Thermo Fisher Scientific, Waltham, MA).","Scaning - Arrays were scanned using the Illumina iScanTM System. raw intensity data were extracted using GenomeStudio software 2.0.5. Quality control checks were performed to assess overall signal intensity, background levels, and internal hybridization controls. Subsequent data processing and normalization were performed in Lumi, an open-source R package, applying variance-stabilizing transformation and robust spline normalization. Differential gene expression (DEG) analysis was conducted using the Limma-Voom R package, with significance thresholds set at an adjusted P< 0.05 and a fold change ≥2.","Nucleic Acid Extraction - Total RNA was extracted using Qiagen RNAeasy kit and submitted for analysis. The quality check was done using nanodrop.","Sample Collection - Tissues were harvested after mice euthanasia and snap frozen in liquid nitrogen. Total RNA was extracted using Qiagen RNAeasy kit and submitted for analysis."],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Rajgopal Govindarajan"],"additional_accession":[]},"is_claimable":false,"name":"ENT3 Knockout Mouse Liver Whole Transcriptome Array","description":"Despite the well-established role of equilibrative nucleoside transporters (ENTs) in salvaging nucleosides for DNA synthesis, the presence of multiple ENT subfamilies within a single genome suggests putative, non-redundant functions in maintaining cellular homeostasis. In this study, we demonstrate that, in contrast to endolysosomal ENT3, which promotes autophagy, cell surface-localized ENT1 is capable of inhibiting autophagy by suppressing AMPK phosphorylation. Consistent with this, silencing ENT1 induces autophagy, whereas silencing ENT3 suppresses it.  ENT1⁻/⁻ and ENT3⁻/⁻ mice display autophagic proficiency and deficiency, respectively. These findings underscore a dynamic and reciprocal regulatory relationship between ENT1 and ENT3 in autophagy, offering new avenues for therapeutic modulation in autophagy-related disorders.","dates":{"release":"2025-11-27T00:00:00Z","modification":"2026-05-27T17:56:19.026Z","creation":"2025-10-29T12:36:36.848Z"},"accession":"E-MTAB-15905","cross_references":{"EFO":["EFO_0002768","EFO_0002944","EFO_0003814","EFO_0003813","EFO_0005518","EFO_0003815"]}}