{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Tianyi Li"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15922"],"description":["The study is to investigate the effects of mono(2-ethylhexyl) phthalate (MEHP) on adult human ovarian tissue at single-cell resolution."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - The libraries were prepared using Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 from 10x genomics.","Sequencing - The libraries were sequenced using the illumina NextSeq2000 platform.","Sample Treatment - Ovarian cortical tissue was cultured on a laminin221-coated insert in a 24-well plate for 6 days. The media was changed every other day. Ovarian tissue was exposed to 0.1% DMSO, 1X MEHP, and 1000X MEHP, respectively."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Raw counts were provided in the data repository. Data analysis was performed using the Seurat normalization.","Sequence Alignment - Single-cell RNA sequencing data were processed using CellRanger 6.1.1 using human reference GRCh38-2020-A."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["NextSeq 2000"],"study_type":["RNA-seq of coding RNA from single cells"],"species":["Homo sapiens"],"pubmed_authors":["Tianyi Li","Pauliina Damdimopoulou"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell atlas of human ovarian response to phthalate exposure reveals glial cell susceptibility and disruption of adhesion and mitochondrial pathways","description":"The study is to investigate the effects of mono(2-ethylhexyl) phthalate (MEHP) on adult human ovarian tissue at single-cell resolution.","dates":{"release":"2026-07-31T00:00:00Z","modification":"2026-07-31T13:26:44.6Z","creation":"2025-10-22T15:21:11.771Z"},"accession":"E-MTAB-15922","cross_references":{"EFO":["EFO_0004170","EFO_0005684","EFO_0004917","EFO_0003816","EFO_0003969","EFO_0004184"]}}