biostudies-arrayexpressTianyi LiHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15922The study is to investigate the effects of mono(2-ethylhexyl) phthalate (MEHP) on adult human ovarian tissue at single-cell resolution.biostudies-arrayexpressLibrary Construction - The libraries were prepared using Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 from 10x genomics.Sequencing - The libraries were sequenced using the illumina NextSeq2000 platform.Sample Treatment - Ovarian cortical tissue was cultured on a laminin221-coated insert in a 24-well plate for 6 days. The media was changed every other day. Ovarian tissue was exposed to 0.1% DMSO, 1X MEHP, and 1000X MEHP, respectively.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Raw counts were provided in the data repository. Data analysis was performed using the Seurat normalization.Sequence Alignment - Single-cell RNA sequencing data were processed using CellRanger 6.1.1 using human reference GRCh38-2020-A.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsNextSeq 2000RNA-seq of coding RNA from single cellsHomo sapiensTianyi LiPauliina DamdimopouloufalseSingle-cell atlas of human ovarian response to phthalate exposure reveals glial cell susceptibility and disruption of adhesion and mitochondrial pathwaysThe study is to investigate the effects of mono(2-ethylhexyl) phthalate (MEHP) on adult human ovarian tissue at single-cell resolution.2026-07-31T00:00:00Z2026-07-31T13:26:44.6Z2025-10-22T15:21:11.771ZE-MTAB-15922EFO_0004170EFO_0005684EFO_0004917EFO_0003816EFO_0003969EFO_0004184