biostudies-arrayexpressLuciano CascioneHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15933We investigated the transcriptomic profiles of non-stimulated samples from day 14 of differentiation with those from day 0biostudies-arrayexpressSequencing - The libraries have been sequenced using HiSeq2000 in the Genomics Facility of the institute of oncology researchSample Collection - sample preparation began with gel digestion of muscle constructs using Trizol (TR118 MRC), followed by phase separation with 1-Bromo-3-chloropropane (B9673).Library Construction - NEBNext UltraExpress RNA Library Prep Kit for Illumina (New England BioLabs Inc.) was employed with the NEBNext Multiplex Oligos for Illumina (New England BioLabs Inc.) and NEBNext® Poly(A) mRNA Magnetic Isolation Module for cDNA synthesis with the addition of barcode sequences.Nucleic Acid Extraction - RNA extraction from muscle cells was then performed according to ReliaPrep™ RNA Miniprep Systems’ protocol (Promega), which included DNase treatment.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to hg38 whole genome using STAR with default paramenters. Genes' Expression have been quantified using HT-Seq count using gencode v22 as gtf.Data Transformation - Read counts have been normalized with TMM algorithm, and converted into log2 counts per million of mapped reads (cpm)MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina HiSeq 2000Image 1.RNA-seq of coding RNAHomo sapiensBiofabrication of a 3D human skeletal muscle microenvironment to study the early steps of fibrosisLuciano CascionefalseTranscriptomics profile of myobundles' maturation levelWe investigated the transcriptomic profiles of non-stimulated samples from day 14 of differentiation with those from day 02025-10-30T00:00:00Z2026-06-16T12:20:49.558Z2025-10-30T15:19:51.583ZE-MTAB-15933ERP183400EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_000418410.1016/j.mtbio.2025.102386