{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["KWANWOO LEE"],"instrument_platform":["Ion Torrent Proton"],"study_type":["RNA-seq of coding RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15958"],"description":["This study aimed to investigate the transcriptomic changes in the mouse brain following oral administration of theogallin. Whole-brain tissues were collected from 13-week-old male mice (C57BL/6J) treated with theogallin or vehicle control. Total RNA was extracted and subjected to RNA sequencing (RNA-seq) to assess differential gene expression associated with theogallin exposure. The dataset provides a foundation for investigating the therapeutic benefits of theogallin in age-associated cognitive disorders. IonXpress_033~036; BC groups, IonXpress_037~040; BT groups"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Whole brain was collected from 13-week-old male mice that were orally administered theogallin for 1 weeks. Brains were rapidly dissected, snap-frozen in liquid nitrogen, and stored at −80°C until RNA extraction.","Library Construction - libraries were prepared with Ion AmpliSeq Transcriptome Mouse Gene Expression Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.","Sequencing - libraries were sequenced on the Ion Proton™ system using IonXpress™ Chip Type P1.1.17 (Thermo Fischer Scientific)","Nucleic Acid Extraction - Total RNA was extracted from whole brain tissues using TRIzol reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions. RNA concentration and integrity were assessed with a NanoDrop spectrophotometer and Agilent Bioanalyzer."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["KWANWOO LEE"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq analysis of whole-brain samples from mice orally administered with Theogallin","description":"This study aimed to investigate the transcriptomic changes in the mouse brain following oral administration of theogallin. Whole-brain tissues were collected from 13-week-old male mice (C57BL/6J) treated with theogallin or vehicle control. Total RNA was extracted and subjected to RNA sequencing (RNA-seq) to assess differential gene expression associated with theogallin exposure. The dataset provides a foundation for investigating the therapeutic benefits of theogallin in age-associated cognitive disorders. IonXpress_033~036; BC groups, IonXpress_037~040; BT groups","dates":{"release":"2025-12-22T00:00:00Z","modification":"2025-12-22T02:01:21.79Z","creation":"2025-11-01T18:15:38.106Z"},"accession":"E-MTAB-15958","cross_references":{"ENA":["ERP183507"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}