biostudies-arrayexpressViktoria SzeifertHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15976We performed a systematic investigation of seven pharmacologic inhibitors (calpeptin, D-pantethine, Y27632, cytochalasin D, GW4869, R5421, and Nexinhib20) on quantitative and qualitative EV properties derived from resting neutrophils. We measured EV numbers, size distribution, total lipid, protein, and RNA content. We also analyzed the EV morphology by cryo-EM and EV cargo qualitatively by mass spectrometry and RNA sequencing (mRNA, miRNA).biostudies-arrayexpressSequencing - Sequencing was performed on an Illumina NovaSeq S4 platform (75 bp single-end). Raw FASTQ files were processed with miRge3.0 software using a custom human miRNA reference built with miRge-build (miRBase). Unique molecular identifiers (12 nt UMIs) were parsed to collapse PCR duplicates.Library Construction - Small RNA libraries were prepared using the Qiagen miRNA Library Prep Kit, which applies bead-based size selection to enrich fragments between 18–40 nt.Nucleic Acid Extraction - Total RNA was extracted using a Qiagen RNA extraction kit, following the manufacturer’s instruction.Sample Collection - For RNA sequencing, EVs were isolated from three biological replicates. The dry EV pellets were resuspended in 10 μL sterile distilled water (nuclease-free), snap frozen, and stored at -80°C until processing within 1 week.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Cleaned reads were aligned and quantified as reads-per-million (RPM). To stabilize variance and enable visualization, values were transformed as log₂(RPM + 1).UnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XmicroRNA profiling by high throughput sequencingHomo sapiensViktoria SzeifertfalseOne way or another: Differential impact of pharmacologic inhibitors on extracellular vesicles derived from resting neutrophilsWe performed a systematic investigation of seven pharmacologic inhibitors (calpeptin, D-pantethine, Y27632, cytochalasin D, GW4869, R5421, and Nexinhib20) on quantitative and qualitative EV properties derived from resting neutrophils. We measured EV numbers, size distribution, total lipid, protein, and RNA content. We also analyzed the EV morphology by cryo-EM and EV cargo qualitatively by mass spectrometry and RNA sequencing (mRNA, miRNA).2025-11-26T00:00:00Z2025-11-26T02:01:54.623Z2025-11-03T16:35:06.8ZE-MTAB-15976ERP183603EFO_0002944EFO_0004170EFO_0002896EFO_0005518EFO_0003816EFO_0004184