{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Kelly Bruton"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15982"],"description":["To study the plasticity of IgE-fated allergen-specific responses, we employed various immunization methods to model type 1 and type 2 immune responses in mice, along with antibody (Ab)-mediated blockade of signaling receptors. Here, we treated epicutaneously sensitized mice with anti-IL-4Ra or an isotype control prior to s.c. reexposures and then performed paired single-cell RNA- and BCR-sequencing on B cells. An additional group of mice were administered cholera toxin and OVA to model a classical type 2 immunization strategy."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Spleens and inguinal lymph nodes were collected one month post-recall response from epicutaneously or subcutaneously sensitized mice and B cells were sorted for sequencing.","Library Construction - Single-cell RNA-sequencing libraries were constructed as per the Chromium Single Cell 5' Reagent Kits User Guide","Nucleic Acid Extraction - Single-cell RNA-sequencing libraries were constructed as per the Chromium Single Cell 5' Reagent Kits User Guide","Sequencing - Libraries were sequenced with NextSeq2000 (Illumina)"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Sequence Alignment - Data were processed with the Cell Ranger Count and V(D)J pipelines (version 7.0.1). Sequencing reads were aligned to the mouse transcriptome (GRCm38).","Data Transformation - Reads associated with retained barcodes were quantified and used to build expression matrices for further analysis with Seurat (version 4.1.3)."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["NextSeq 2000"],"study_type":["RNA-seq of coding RNA from single cells"],"species":["Mus musculus"],"pubmed_authors":["Kelly Bruton"],"additional_accession":[]},"is_claimable":false,"name":"Pathogenic IgE-fated memory B cell responses retain functional plasticity","description":"To study the plasticity of IgE-fated allergen-specific responses, we employed various immunization methods to model type 1 and type 2 immune responses in mice, along with antibody (Ab)-mediated blockade of signaling receptors. Here, we treated epicutaneously sensitized mice with anti-IL-4Ra or an isotype control prior to s.c. reexposures and then performed paired single-cell RNA- and BCR-sequencing on B cells. An additional group of mice were administered cholera toxin and OVA to model a classical type 2 immunization strategy.","dates":{"release":"2025-11-13T00:00:00Z","modification":"2026-05-27T16:52:25.36Z","creation":"2025-11-06T11:29:26.113Z"},"accession":"E-MTAB-15982","cross_references":{"ENA":["ERP183685"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005684","EFO_0004917","EFO_0005518","EFO_0003816","EFO_0004184"]}}