biostudies-arrayexpressKelly BrutonMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15982To study the plasticity of IgE-fated allergen-specific responses, we employed various immunization methods to model type 1 and type 2 immune responses in mice, along with antibody (Ab)-mediated blockade of signaling receptors. Here, we treated epicutaneously sensitized mice with anti-IL-4Ra or an isotype control prior to s.c. reexposures and then performed paired single-cell RNA- and BCR-sequencing on B cells. An additional group of mice were administered cholera toxin and OVA to model a classical type 2 immunization strategy.biostudies-arrayexpressSample Collection - Spleens and inguinal lymph nodes were collected one month post-recall response from epicutaneously or subcutaneously sensitized mice and B cells were sorted for sequencing.Library Construction - Single-cell RNA-sequencing libraries were constructed as per the Chromium Single Cell 5' Reagent Kits User GuideNucleic Acid Extraction - Single-cell RNA-sequencing libraries were constructed as per the Chromium Single Cell 5' Reagent Kits User GuideSequencing - Libraries were sequenced with NextSeq2000 (Illumina)OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - Data were processed with the Cell Ranger Count and V(D)J pipelines (version 7.0.1). Sequencing reads were aligned to the mouse transcriptome (GRCm38).Data Transformation - Reads associated with retained barcodes were quantified and used to build expression matrices for further analysis with Seurat (version 4.1.3).MetabolomicsUnknownTranscriptomicsGenomicsProteomicsNextSeq 2000RNA-seq of coding RNA from single cellsMus musculusKelly BrutonfalsePathogenic IgE-fated memory B cell responses retain functional plasticityTo study the plasticity of IgE-fated allergen-specific responses, we employed various immunization methods to model type 1 and type 2 immune responses in mice, along with antibody (Ab)-mediated blockade of signaling receptors. Here, we treated epicutaneously sensitized mice with anti-IL-4Ra or an isotype control prior to s.c. reexposures and then performed paired single-cell RNA- and BCR-sequencing on B cells. An additional group of mice were administered cholera toxin and OVA to model a classical type 2 immunization strategy.2025-11-13T00:00:00Z2026-05-27T16:52:25.36Z2025-11-06T11:29:26.113ZE-MTAB-15982ERP183685EFO_0002944EFO_0004170EFO_0005684EFO_0004917EFO_0005518EFO_0003816EFO_0004184