{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Bei Wei"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15983"],"description":["The 5’ single-cell library was prepared strictly following the manufacturer's instructions (User Guide CG000733, 10x Genomics) using the Chromium GEM-X Single Cell 5' Kit v3 (cat# PN-1000701)."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - The fresh cervical cancer samples were collected and processed immediately with the procedure modified from the manufacturer's protocol (Demonstrated Protocol, CG000147).","Sample Collection - The cervical cancer samples, including 8 cases of adenocarcinoma, 6 cases of squamous cell carcinoma, and 2 cases of adenosquamous carcinoma, were obtained from multiple sites, including Sun Yat-sen Memorial Hospital of Sun Yat-sen University, the First Hospital of Jilin University and the Third Affiliated Hospital of Harbin Medical University.","Library Construction - The 5’ single-cell library was prepared strictly following the manufacturer's instructions (User Guide CG000733, 10x Genomics) using the Chromium GEM-X Single Cell 5' Kit v3 (cat# PN-1000699).","Sequencing - The final libraries were pooled and sequenced at the Illumina NovaSeq6000 sequencer using the 150 bp PE kits (read 1 = 26 bp, i7 index = 10 bp, i5 index = 10 bp, read 2 = 90 bp read configuration) targeting an average of 20,000 reads per cell."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Raw data were processed using the 10x Genomics Cell Ranger pipeline (v7.1.0)."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA from single cells"],"species":["Homo sapiens"],"pubmed_title":["Single-Cell and Spatial Transcriptomic Profiling Reveals Distinct Tumor Microenvironment Dynamics in Cervical Adenocarcinoma and Squamous Cell Carcinoma"],"pubmed_authors":["Yaqin Wu# 1, Bei Wei# 2,6, Zhentong Wei# 3, Hongyan Wang4, Yunyun Liu5, Dongdong Xu5, Xiaokang Li2, Liyuan Guo4, Huaiwu Lu5,6, and Huiling Shang1,6","Bei Wei"],"additional_accession":[]},"is_claimable":false,"name":"Single-Cell and Spatial Transcriptomic Profiling Reveals Distinct Tumor Microenvironment Dynamics in Cervical Adenocarcinoma and Squamous Cell Carcinoma","description":"The 5’ single-cell library was prepared strictly following the manufacturer's instructions (User Guide CG000733, 10x Genomics) using the Chromium GEM-X Single Cell 5' Kit v3 (cat# PN-1000701).","dates":{"release":"2025-11-07T00:00:00Z","modification":"2026-05-27T14:27:39.774Z","creation":"2025-11-06T10:52:34.31Z"},"accession":"E-MTAB-15983","cross_references":{"EFO":["EFO_0002944","EFO_0004170","EFO_0005684","EFO_0005518","EFO_0003816","EFO_0004184"]}}