biostudies-arrayexpressBei WeiHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15983The 5’ single-cell library was prepared strictly following the manufacturer's instructions (User Guide CG000733, 10x Genomics) using the Chromium GEM-X Single Cell 5' Kit v3 (cat# PN-1000701).biostudies-arrayexpressNucleic Acid Extraction - The fresh cervical cancer samples were collected and processed immediately with the procedure modified from the manufacturer's protocol (Demonstrated Protocol, CG000147).Sample Collection - The cervical cancer samples, including 8 cases of adenocarcinoma, 6 cases of squamous cell carcinoma, and 2 cases of adenosquamous carcinoma, were obtained from multiple sites, including Sun Yat-sen Memorial Hospital of Sun Yat-sen University, the First Hospital of Jilin University and the Third Affiliated Hospital of Harbin Medical University.Library Construction - The 5’ single-cell library was prepared strictly following the manufacturer's instructions (User Guide CG000733, 10x Genomics) using the Chromium GEM-X Single Cell 5' Kit v3 (cat# PN-1000699).Sequencing - The final libraries were pooled and sequenced at the Illumina NovaSeq6000 sequencer using the 150 bp PE kits (read 1 = 26 bp, i7 index = 10 bp, i5 index = 10 bp, read 2 = 90 bp read configuration) targeting an average of 20,000 reads per cell.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Raw data were processed using the 10x Genomics Cell Ranger pipeline (v7.1.0).MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNA from single cellsHomo sapiensSingle-Cell and Spatial Transcriptomic Profiling Reveals Distinct Tumor Microenvironment Dynamics in Cervical Adenocarcinoma and Squamous Cell CarcinomaYaqin Wu# 1, Bei Wei# 2,6, Zhentong Wei# 3, Hongyan Wang4, Yunyun Liu5, Dongdong Xu5, Xiaokang Li2, Liyuan Guo4, Huaiwu Lu5,6, and Huiling Shang1,6Bei WeifalseSingle-Cell and Spatial Transcriptomic Profiling Reveals Distinct Tumor Microenvironment Dynamics in Cervical Adenocarcinoma and Squamous Cell CarcinomaThe 5’ single-cell library was prepared strictly following the manufacturer's instructions (User Guide CG000733, 10x Genomics) using the Chromium GEM-X Single Cell 5' Kit v3 (cat# PN-1000701).2025-11-07T00:00:00Z2026-05-27T14:27:39.774Z2025-11-06T10:52:34.31ZE-MTAB-15983EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0003816EFO_0004184