{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Chantal Wientjens"],"instrument_platform":["Illumina HiSeq 2500"],"study_type":["RNA-seq of coding RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-15989"],"description":["Type 2 Innate Lymphoid cells (ILC2)  play a crucial role in allergic airway inflammation. To identify transcriptional differences between naive ILC2 and allergen induced ILC2, mice were challenged with PBS control or papain on days 0, 3, 6 and 13. On day 14 ILC2 were isolated, sort-purified and sequenced."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - RNA was isolated with Trizol.","Sample Collection - Lungs were isolated from the mice and processed to obtain cells in a single cell suspension. ILC2 were sorted by FACS.","Sequencing - Library preparation was performed using the QuantSeq 3´ mRNA-Seq Library Prep kit (Lexogen) according to manufacturer´s instructions.","Library Construction - Library preparation was performed using the QuantSeq 3´ mRNA-Seq Library Prep kit (Lexogen) according to manufacturer´s instructions."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Christoph Wilhelm","Chantal Wientjens"],"additional_accession":[]},"is_claimable":false,"name":"Bulk RNA-Seq of lung Type 2 Innate Lymphoid cells (ILC2) in response to papain","description":"Type 2 Innate Lymphoid cells (ILC2)  play a crucial role in allergic airway inflammation. To identify transcriptional differences between naive ILC2 and allergen induced ILC2, mice were challenged with PBS control or papain on days 0, 3, 6 and 13. On day 14 ILC2 were isolated, sort-purified and sequenced.","dates":{"release":"2025-12-10T00:00:00Z","modification":"2025-12-10T02:01:20.371Z","creation":"2025-11-06T11:39:06.383Z"},"accession":"E-MTAB-15989","cross_references":{"ENA":["ERP183696"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}