biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsChantal WientjensIllumina HiSeq 2500RNA-seq of coding RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-15989Type 2 Innate Lymphoid cells (ILC2) play a crucial role in allergic airway inflammation. To identify transcriptional differences between naive ILC2 and allergen induced ILC2, mice were challenged with PBS control or papain on days 0, 3, 6 and 13. On day 14 ILC2 were isolated, sort-purified and sequenced.biostudies-arrayexpressNucleic Acid Extraction - RNA was isolated with Trizol.Sample Collection - Lungs were isolated from the mice and processed to obtain cells in a single cell suspension. ILC2 were sorted by FACS.Sequencing - Library preparation was performed using the QuantSeq 3´ mRNA-Seq Library Prep kit (Lexogen) according to manufacturer´s instructions.Library Construction - Library preparation was performed using the QuantSeq 3´ mRNA-Seq Library Prep kit (Lexogen) according to manufacturer´s instructions.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesChristoph WilhelmChantal WientjensfalseBulk RNA-Seq of lung Type 2 Innate Lymphoid cells (ILC2) in response to papainType 2 Innate Lymphoid cells (ILC2) play a crucial role in allergic airway inflammation. To identify transcriptional differences between naive ILC2 and allergen induced ILC2, mice were challenged with PBS control or papain on days 0, 3, 6 and 13. On day 14 ILC2 were isolated, sort-purified and sequenced.2025-12-10T00:00:00Z2025-12-10T02:01:20.371Z2025-11-06T11:39:06.383ZE-MTAB-15989ERP183696EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184