biostudies-arrayexpressChen Pei-yunTrichomonas vaginalisCLC, DeSeq2https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16002We investigated the effects of B. breve on T. vaginalis growth, gene expression, and host-pathogen interactions. Transcriptomic analyses were performed to characterize T. vaginalis responses to B. breve.biostudies-arrayexpressSequencing - Knockdown and control samples were sequenced together in one flowcell on one and two lanes, respectively.Sample Treatment - Co-culture experiments were conducted to investigate the direct interactions between T. vaginalis and B. breve. Mid-log phase bacteria were standardized to 10⁸ CFU, and parasites were prepared at 10⁷ cells. Both organisms were combined in serum-free YI-S and incubated anaerobically for four hours.Sample Treatment - T. vaginalis only culture in serum-free YI-S and incubated anaerobically for four hours.Library Construction - RNA-seq libraries were constructed using the Illumina Poly(A) Capture (Cat. No. 20777928), cDNA Synthesis (Cat. No. 20761354), and RNA Prep, Ligation (Cat. No. 20778789) kits according to the manufacturer’s protocols.Sample Collection - T. vaginalis co-cultured with B. breve or T. vaginalis alone (negative control) were transferred into centrifuge tubes and centrifuged at 2500 rpm for 5 minutes. The supernatant was removed, and TRIzol reagent was added and mixed thoroughly.Nucleic Acid Extraction - Total RNA was extracted using the Quick-RNA MiniPrep Kit (Zymo Research, Cat. No. R2052) according to the manufacturer’s instructions. Briefly, cells were lysed in RNA Lysis Buffer, and the lysates were passed through spin columns for RNA binding. After on-column DNase I digestion to remove genomic DNA, the columns were washed and RNA was eluted in RNase-free water. RNA concentration and purity were determined using a NanoDrop spectrophotometer, and RNA integrity was verified with an Agilent Bioanalyzer.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - TPM value was analyzedMetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XRNA-seq of coding RNATrichomonas vaginalisChen Pei-yunfalseBifidobacterium breve Enhances Growth and Alters the Transcriptome of Trichomonas vaginalis With Transient Modulation of Host Inflammatory ResponsesWe investigated the effects of B. breve on T. vaginalis growth, gene expression, and host-pathogen interactions. Transcriptomic analyses were performed to characterize T. vaginalis responses to B. breve.2025-11-18T00:00:00Z2026-05-27T18:06:19.091Z2025-11-06T12:02:50.913ZE-MTAB-16002ERP183806EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0003969EFO_0004184