biostudies-arrayexpressTwan van den BeuckenHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16022The purpose of this experiment was to generate an RNAseq dataset of human cardiomyocytes with lower levels of FOXO3 exposed to doxorubicin for 24 hours. Stable knock-down of FOXO3 was achieved using lentiviral shRNA construct. As negative control cells bearing an shRNA targeting GFP was usedbiostudies-arrayexpressLibrary Construction - Poly(A) libraries were generated using the Zephyr G3® NGS automated system with the NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0 (NOVA-5198–02, PerkinElmer). Library preparation included NEXTFLEX® Poly(A) Beads 2.0 (NOVA-512992, PerkinElmer) for mRNA enrichment and NEXTFLEX® Unique Dual Index Barcodes (NOVA-512923, PerkinElmer) for sample multiplexing. Each library was prepared using 100 ng of total RNA as input, with RNA integrity numbers (RIN ≥ 8). A total of 13 PCR cycles were performed during library amplification.Sequencing - Sequencing was carried out on the Illumina NovaSeq 6000 using an S2 flow cell, generating paired-end reads with 100 cycles per read.Sample Collection - After the exposure, cells were washed once with PBS and subsequently lysed in 0.5 mL QIAzol Lysis Reagent (79306, Qiagen).Nucleic Acid Extraction - Total RNA was extracted using chloroform and isopropanol precipitation.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Raw sequencing reads were quality-checked and aligned to the reference genome using STAR. Gene-level count matrices were generated and imported into DESeq2 for normalization. Size-factor normalization was performed using the median-of-ratios method to correct for library size and compositional differences.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAHomo sapiensTwan van den BeuckenfalseRNA-seq of of doxorubicin exposed SV40 immortalized human cardiomyocytes with FOXO3 knock-down or shGFP controlThe purpose of this experiment was to generate an RNAseq dataset of human cardiomyocytes with lower levels of FOXO3 exposed to doxorubicin for 24 hours. Stable knock-down of FOXO3 was achieved using lentiviral shRNA construct. As negative control cells bearing an shRNA targeting GFP was used2025-12-29T00:00:00Z2026-05-27T16:04:21.572Z2025-11-13T14:29:04.81ZE-MTAB-16022ERP183898EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184