biostudies-arrayexpressCarl MANNHomo sapiensSalmon and tximetaEdgeR and Limmahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16030Transcriptomic analysis of inflammatory gene expression in a human mammary fibroblast cell line in proliferation and IR-induced senescence, with and without IL1-alpha treatment (10 pg/ml for 24 hours). Cells were induced into senescence by irradiation with 40 Gy of 120 kV X-rays and then incubated 9 days before treating with IL1-alpha.biostudies-arrayexpressSequencing - Paired-End 42 nucleotides in an Illumina NextSeq 500 sequencer.Growth Protocol - DMEM + glutamine + pyruvate + pen/strep in 5% O2 + 5% CO2 incubator.Nucleic Acid Extraction - RNA was extracted with the Macherey-Nagel Nucleospin RNA Plus.Sample Collection - Cell monolayers were trypsinized, neutralized with medium, washed with PBS, and centrifuged. Washed cell pellets were resuspended in Macherey-Nagel Nucleospin RNA Plus Lysis Buffer.Library Construction - NEBNext Ultra II Directional RNA Library Kit with PolyA RNA selectionSample Treatment - Proliferating cells were treated with 10 pg/ml Interleukin-alpha (Sigma I2778)for 24 hours. M168 cells were induced into senescence by irradiating with 40 Gy of 120 KV X-rays produced by a Faxitron 43855D generator. Cells were incubated for 10 days post irradiation before harvesting for RNA isolation. For IL1-alpha treatment of irradiated cells, the cells were irradiated with 40 Gy of 120 KV X-rays as above and cells were incubated for 9 days and then treated for 24 hours with 10 pg/ml of IL1-alpha (Sigma I2778).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesData Transformation - Data were normalized with EdgeR and Limma voomSequence Alignment - Fastq sequences were mappled to the human transcriptome using the Salmon transcriptome alignment software.UnknownTranscriptomicsGenomicsProteomicsNextSeq 550RNA-seq of coding RNAHomo sapiensCarl MANNfalseRNA-seq of a human mammary fibroblast cell line in proliferation and IR-induced senescence with and without IL1-alpha treatment.Transcriptomic analysis of inflammatory gene expression in a human mammary fibroblast cell line in proliferation and IR-induced senescence, with and without IL1-alpha treatment (10 pg/ml for 24 hours). Cells were induced into senescence by irradiation with 40 Gy of 120 kV X-rays and then incubated 9 days before treating with IL1-alpha.2026-01-01T00:00:00Z2026-01-02T02:03:17.382Z2025-11-06T12:48:18.069ZE-MTAB-16030ERP183775E-MTAB-14265E-MTAB-14201EFO_0002944EFO_0004170EFO_0003789EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184EFO_0003969