{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["KIYOUNG EUN"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of total RNA"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16032"],"description":["Through a metabolic RNAi-based screen for cell invasion, potential metastatic functions of AASS were identified. This study was to investigate the role of AASS and its metabolic product, α-aminoadipate (2-AAA), in the metastatic potential of lung adenocarcinoma. H1299 lung adenocarcinoma cells were infected with a pLKO.1-based lentiviral vector carrying shRNA targeting AASS. Poly-A RNA sequencing was then performed on control and AASS-knockdown H1299 cells to assess differentially expressed genes modulated by AASS depletion."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - Approximately 2 ug of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). Following purification, the poly(A) mRNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with a strand-specific library preparation by dUTP method. The average insert size for the paired-end libraries was 300±50 bp.","Sample Collection - Cells were collected by Trypsization and washed with PBS twice.","Nucleic Acid Extraction - After cell collection, total RNA was extracted using the RNeasy Kit (QIAGEN) according to the manufacturer’s instructions.","Sequencing - RNAseq was performed by LC Sciences (Huston, TX, Unites States) through the Illumina NovaSeq 6000 platform.","Growth Protocol - All cells were cultured in RPMI medium supplemented with 10% FBS and 1% penicillin–streptomycin."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["KIYOUNG EUN"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptomic analysis of AASS-knockdown H1299 cells to identify its role in metastatic potential of lung adenocarcinoma","description":"Through a metabolic RNAi-based screen for cell invasion, potential metastatic functions of AASS were identified. This study was to investigate the role of AASS and its metabolic product, α-aminoadipate (2-AAA), in the metastatic potential of lung adenocarcinoma. H1299 lung adenocarcinoma cells were infected with a pLKO.1-based lentiviral vector carrying shRNA targeting AASS. Poly-A RNA sequencing was then performed on control and AASS-knockdown H1299 cells to assess differentially expressed genes modulated by AASS depletion.","dates":{"release":"2026-02-28T00:00:00Z","modification":"2026-02-28T02:02:14.844Z","creation":"2025-11-04T09:36:25.163Z"},"accession":"E-MTAB-16032","cross_references":{"ENA":["ERP183656"],"EFO":["EFO_0002944","EFO_0004170","EFO_0009653","EFO_0003789","EFO_0005518","EFO_0004184"]}}