biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsKIYOUNG EUNIllumina NovaSeq 6000RNA-seq of total RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16032Through a metabolic RNAi-based screen for cell invasion, potential metastatic functions of AASS were identified. This study was to investigate the role of AASS and its metabolic product, α-aminoadipate (2-AAA), in the metastatic potential of lung adenocarcinoma. H1299 lung adenocarcinoma cells were infected with a pLKO.1-based lentiviral vector carrying shRNA targeting AASS. Poly-A RNA sequencing was then performed on control and AASS-knockdown H1299 cells to assess differentially expressed genes modulated by AASS depletion.biostudies-arrayexpressLibrary Construction - Approximately 2 ug of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). Following purification, the poly(A) mRNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with a strand-specific library preparation by dUTP method. The average insert size for the paired-end libraries was 300±50 bp.Sample Collection - Cells were collected by Trypsization and washed with PBS twice.Nucleic Acid Extraction - After cell collection, total RNA was extracted using the RNeasy Kit (QIAGEN) according to the manufacturer’s instructions.Sequencing - RNAseq was performed by LC Sciences (Huston, TX, Unites States) through the Illumina NovaSeq 6000 platform.Growth Protocol - All cells were cultured in RPMI medium supplemented with 10% FBS and 1% penicillin–streptomycin.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesKIYOUNG EUNfalseTranscriptomic analysis of AASS-knockdown H1299 cells to identify its role in metastatic potential of lung adenocarcinomaThrough a metabolic RNAi-based screen for cell invasion, potential metastatic functions of AASS were identified. This study was to investigate the role of AASS and its metabolic product, α-aminoadipate (2-AAA), in the metastatic potential of lung adenocarcinoma. H1299 lung adenocarcinoma cells were infected with a pLKO.1-based lentiviral vector carrying shRNA targeting AASS. Poly-A RNA sequencing was then performed on control and AASS-knockdown H1299 cells to assess differentially expressed genes modulated by AASS depletion.2026-02-28T00:00:00Z2026-02-28T02:02:14.844Z2025-11-04T09:36:25.163ZE-MTAB-16032ERP183656EFO_0002944EFO_0004170EFO_0009653EFO_0003789EFO_0005518EFO_0004184