biostudies-arrayexpressKevin SchmidtHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16034This study aimed to explore the therapeutic potential of the cysteine protease inhibitor aloxistatin (E64d) as a candidate to combat cardaic fibrotic progression. Human cardiac fibroblasts (HCFs) derived from diseased myocardium including ischemic and dilated cardiomyopathies (ICM, DCM) were treated for 48 h with 100 µM aloxistatin or DMSO control and pro-fibrotic stimulated with 5 ng/mL TGFβ1 or respective vehicle (veh) control (4 mM HCl containing 0.1% bovine serum albumin). HCF were harvested with QiAzol Lysis reagent (Qiagen) and RNA was isolated based on phenol-chloroform-extraction procedure.biostudies-arrayexpressNucleic Acid Extraction - RNA was isolated based on phenol-chloroform-extraction procedure.Library Construction - polyA enrichment library preparation was performed by NovogeneSample Collection - HCF were harvested with QiAzol Lysis reagent (Qiagen)Sequencing - Illumina (NovaSeq X Plus) platform was used for paired end 150 bp sequencing (30 M reads per sample). Sequencing was done by Novogene.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - DESeq2 pipeline (for R) was used for count normalization and DEG calculation with default parametersSequence Alignment - mapping to GRCh38 with STAR aligner using default parametersMetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XRNA-seq of coding RNAHomo sapiensMaria JordanKevin Schmidtjan FiedlerfalsemRNA-seq of human cardiac fibrobasts (HCF) profibrotic stimulated with TGFb and treated with aloxistatin (E64d)This study aimed to explore the therapeutic potential of the cysteine protease inhibitor aloxistatin (E64d) as a candidate to combat cardaic fibrotic progression. Human cardiac fibroblasts (HCFs) derived from diseased myocardium including ischemic and dilated cardiomyopathies (ICM, DCM) were treated for 48 h with 100 µM aloxistatin or DMSO control and pro-fibrotic stimulated with 5 ng/mL TGFβ1 or respective vehicle (veh) control (4 mM HCl containing 0.1% bovine serum albumin). HCF were harvested with QiAzol Lysis reagent (Qiagen) and RNA was isolated based on phenol-chloroform-extraction procedure.2026-07-06T00:00:00Z2026-07-06T15:05:10.4Z2025-11-13T14:50:12.194ZE-MTAB-16034ERP183922EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184