biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsLi LiRNA-seq of total RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16037Expression levels of 770 fibrosis-related genes were measured with the nCounter SPRINT Profiler using the human fibrosis panel V2 assay (NanoString Technologies, Munich, Germany).biostudies-arrayexpressScaning - Hybridized samples were processes and scanned on nanostring nCounter Spinter Profiler according to the manufacturer's instructions.Labeling - RNA samples were hybridized with reporter and capture probes from the Nanostring Human Fibrosis Panel V2 following the standard nCounter protocol.Nucleic Acid Extraction - 10µl of 2-mercaptoethanol was added to 1ml of lysis buffer taken from a PureLink RNA Mini Kit. Medium was discarded, and the cells were washed 2 times with cold PBS. 350µl of lysis buffer work solution was then added to the cells in one well of a 6 well plate. After incubating for 3 minutes, the cells were scratched lose and the lysate was transferred into an Eppendorf tube for RNA isolation. RNA was isolated using the PureLink RNA Mini Kit following the manufacturer’s instructions. RNA was eluated in 35μl of RNase free water.Hybridization - Hybridization was performed at 65 degree for 16/18 hours accroding to the Nanostring nCounter standard protocol.Sample Collection - Human immortalized N/TERT-1 keratinocytes and N/TERT-1-derived cell lines were maintained in K-sfm medium supplemented with 1% penicillin, human recombinant epidermal growth factor (hrEGF) and bovine pituitary extract (BPE) at 37°C, 5% CO2. N/TERT-1 keratinocytes with Dox-inducible DKK3 overexpression and Wnt reporter were starved for 24 h in supplement-free K-sfm medium and then stimulated with 0.2 ug/ml doxycycline. DKK3 was knocked down in N/TERT-1 with 20 nM DKK3 siRNA (GeneGlobe ID SI00100933) using Hyperfect transfection reagent including non-targeting siRNA and mock-transfection as controls. 24 h after Dox induction or siRNA transfection, RNA was extracted for further analysis.MIAME ScoreRaw DataOrganizationAssays and DataMAGE-TAB FilesArray DesignsLi LifalseThe effect of DKK3 expression on fibrosis related genes in human keratinocytesExpression levels of 770 fibrosis-related genes were measured with the nCounter SPRINT Profiler using the human fibrosis panel V2 assay (NanoString Technologies, Munich, Germany).2025-11-17T00:00:00Z2025-11-27T10:35:18.269Z2025-11-03T15:03:07.501ZE-MTAB-16037EFO_0002944EFO_0003814EFO_0003813EFO_0009653EFO_0005518EFO_0003815