biostudies-arrayexpressSanushi DambureRattus norvegicushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16042Chronic kidney disease (CKD), affecting over 800million individuals globally, causes a distinct cardiorenal HFpEF phenotype, characterised by metabolic, structural and functional remodelling. Sotagliflozin (SOTA), a dual sodium-glucose co-transporter (SGLT) 1 and 2 inhibitor, has demonstrated superior efficacy in reducing cardiovascular events in CKD patients. This study investigates impact of SOTA treatment on cardiac genomic profile in CKD. The study included three groups (n = 5 per group): Sham, CKD, and SOTA-treated CKD. CKD was surgically induced in male Wistar rats (n = 10) using 5/6 nephrectomy for a period of 4 weeks, with SOTA treatment (5 mg/kg/day) administered for 3 weeks. Bulk RNA sequencing was performed on cardiac tissue harvested at experimental endpoint.biostudies-arrayexpressSample Collection - Hearts harvested at the experimental endpoint and snap-frozen in liquid nitrogen, were stored at −80 °C until RNA extractionNucleic Acid Extraction - BGI RNA extraction protocol as completed by BGI Genomics Co Ltd using tandard Sensitivity RNAAnalysis Kit (15 nt) (DNF-471)Library Construction - RNA extracted from snap frozen cardiac tissue (n=5) CKD, Sham, Sota was used for library preperation. mRNA enrichment was performed on total RNA using oligo(dT)-attached magnetic beads. The enriched mRNA with poly(A) tails was fragmented using a fragmentation buffer, followed by reverse transcription using random N6 primers to synthesize cDNA double strands. The synthesized double stranded DNA was then end-repaired and 5'-phosphorylated, with a protruding 'A' at the 3' end forming a blunt end, followed by ligation of a bubble-shaped adapter with a protruding 'T' at the 3' end. The ligation products were PCR amplified using specific primers. The PCR products were denatured to single strands, and then single-stranded circular DNA libraries were generated using a bridged primer.Sequencing - The constructed libraries were quality-checked and sequenced after passing the quality control. Sequencing was performed on DNBSEQ platform with PE100 read length.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - Trimmed reads were aligned to the Rattus norvegicus reference genome (mRatBN7.2, Ensembl release 113) using HISAT2 v2.2.1 with default parameters. SAMtools v1.19.2 was used to convert, sort, and index the alignment files into BAM files.Data Transformation - Raw reads were assessed using FastQC v0.12.1, and summaries were compiled with MultiQC v1.28. Adapter and low-quality sequences were removed using Trimmomatic v0.39 with trimming parameters SLIDINGWINDOW:4:20, MINLEN:20. Post-trimming quality was re-evaluated with FastQC v0.12.1, and the results were consolidated using MultiQC v1.29.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsDNBSEQ-G400RNA-seq of coding RNARattus norvegicusSanushi DamburefalseImpact of dual SGLT1/2 inhibitor Sotagliflozin on the cardiac genetic profile in cardiorenal heart failure with preserved ejection fraction (HFpEF)Chronic kidney disease (CKD), affecting over 800million individuals globally, causes a distinct cardiorenal HFpEF phenotype, characterised by metabolic, structural and functional remodelling. Sotagliflozin (SOTA), a dual sodium-glucose co-transporter (SGLT) 1 and 2 inhibitor, has demonstrated superior efficacy in reducing cardiovascular events in CKD patients. This study investigates impact of SOTA treatment on cardiac genomic profile in CKD. The study included three groups (n = 5 per group): Sham, CKD, and SOTA-treated CKD. CKD was surgically induced in male Wistar rats (n = 10) using 5/6 nephrectomy for a period of 4 weeks, with SOTA treatment (5 mg/kg/day) administered for 3 weeks. Bulk RNA sequencing was performed on cardiac tissue harvested at experimental endpoint.2026-08-31T00:00:00Z2026-08-31T01:00:38.502Z2025-11-13T13:44:04.871ZE-MTAB-16042ERP183967EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184