{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Enrique Rosell Moll"],"organism":["Oncorhynchus mykiss"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16058"],"description":["The aim of this study was to characterize different adipose tissue depots in rainbow trout (Oncorhynchus mykiss) through the analysis of mRNA and miRNA expression profiles. Mature adipocytes were isolated from visceral (VAT), subcutaneous (SAT), and intramuscular (IMAT) adipose tissues, and RNA sequencing (RNA-seq) was performed. Data were curated, quality-checked, and filtered prior to downstream analyses. This dataset contains the FASTQ files generated in the study. The results will contribute to a better understanding of the molecular differences and similarities among adipose depots in fish reared under standard aquaculture conditions, providing a foundation for improving adiposity control, animal health, fillet yield, and overall product quality."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - Libraries were sequenced by Azenta/GENEWIZ (Leipzig, Germany) on an Illumina NovaSeq platform using a 2×150 bp paired-end configuration, generating approximately 20 million paired-end reads per sample. Quality control ensured that at least 80% of bases had Q-scores ≥ Q30.","Sample Collection - Adipose tissue was dissected from fish (visceral, subcutaneous, and intramuscular depots). Mature adipocytes were isolated by mechanical and enzymatic digestion using collagenase type II.","Nucleic Acid Extraction - Total RNA was extracted using TRIzol reagent (Thermo Fisher Scientific, Waltham, MA, USA) followed by isopropanol, chloroform, and ethanol extraction according to the manufacturer’s instructions.","Library Construction - RNA libraries were prepared by Azenta/GENEWIZ (Leipzig, Germany) using Illumina RNA library preparation with poly(A) selection according to the company’s standard protocol."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Raw counts were normalized using the default size-factor method implemented in DESeq2, which accounts for differences in library size among samples."],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["not applicable","Illumina NovaSeq 6000"],"study_type":["RNA-seq of total RNA"],"species":["Oncorhynchus mykiss"],"pubmed_title":["Analysing the transcriptomic diversity in isolated mature adipocytes from the major fat depots in rainbow trout (Oncorhynchus mykiss)"],"pubmed_authors":["Enrique Rosell Moll","E. Rosell-Moll, E. Capilla, I. Navarro, D. Garcia de la serrana"],"additional_accession":[]},"is_claimable":false,"name":"Rainbow trout adipose tissue depots (VAT, SAT, IMAT) - mRNAs","description":"The aim of this study was to characterize different adipose tissue depots in rainbow trout (Oncorhynchus mykiss) through the analysis of mRNA and miRNA expression profiles. Mature adipocytes were isolated from visceral (VAT), subcutaneous (SAT), and intramuscular (IMAT) adipose tissues, and RNA sequencing (RNA-seq) was performed. Data were curated, quality-checked, and filtered prior to downstream analyses. This dataset contains the FASTQ files generated in the study. The results will contribute to a better understanding of the molecular differences and similarities among adipose depots in fish reared under standard aquaculture conditions, providing a foundation for improving adiposity control, animal health, fillet yield, and overall product quality.","dates":{"release":"2026-01-12T00:00:00Z","modification":"2026-01-12T02:02:17.667Z","creation":"2025-11-18T17:49:04.96Z"},"accession":"E-MTAB-16058","cross_references":{"ENA":["ERP185267"],"EFO":["EFO_0002944","EFO_0004170","EFO_0009653","EFO_0005518","EFO_0003816","EFO_0004184"]}}