biostudies-arrayexpressQian Yee WooHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16059RNA-seq profiling was performed on MCF7-EV (empty vector control), MCF7-PRB, MCF7-PRB-FFF, and MCF7-PRB-QQQ cells following 6 hours of treatment with one of four conditions: ethanol (C), R5020 (R), estrogen (E2), or estrogen plus R5020 (E2R).biostudies-arrayexpressLibrary Construction - . DNA free RNA samples were submitted to Genome Institute of Singapore (Agency for Science, Technology and Research) for library preparation and paired-end sequencing using Illumina HiSeq4000 platform.Sample Collection - Following hormone treatment, the culture media in 60-mm dishes were removed thoroughly. 1ml of TRIzol was added to each plate for cell lysis.Nucleic Acid Extraction - The cultured cells were lysed in cold TRIzol reagent, and total RNA was isolated according to the manufacturer’s protocol (Life Technologies, MD, USA). RNA was precipitated using isopropanol and pelleted at 12000xg, 4°C for 15 minutes. The pelleted RNA was washed twice with 75% ethanol prepared in DEPC-treated water, briefly air-dried and resuspended in DEPC-treated water.Sequencing - . DNA free RNA samples were submitted to Genome Institute of Singapore (Agency for Science, Technology and Research) for library preparation and paired-end sequencing using Illumina HiSeq4000 platform.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Differential gene expression analysis was performed using DESeq2 package in Rstudio.Sequence Alignment - Sequencing read quality was assessed using FastQC. Transcription quantification was performed using the STAR aligners against the human reference genome GRCh38 (hg38) obtained from Ensembl. Transcript-level counts were summarized to gene-level counts using R package tximport. Differential gene expression analysis was performed using DESeq2.UnknownTranscriptomicsGenomicsProteomicsIllumina HiSeq 4000RNA-seq of total RNAHomo sapiensQian Yee WoofalseMethylation mimic mutations of progesterone receptor AF1 impair gene-specific regulation through stabilized chromatin interactionsRNA-seq profiling was performed on MCF7-EV (empty vector control), MCF7-PRB, MCF7-PRB-FFF, and MCF7-PRB-QQQ cells following 6 hours of treatment with one of four conditions: ethanol (C), R5020 (R), estrogen (E2), or estrogen plus R5020 (E2R).2026-03-31T00:00:00Z2026-03-31T01:03:46.76Z2025-11-13T14:37:43.897ZE-MTAB-16059ERP184112EFO_0002944EFO_0004170EFO_0009653EFO_0004917EFO_0005518EFO_0003816EFO_0004184