{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Conor Templeton"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16071"],"description":["Differentially m6A modified transcripts were identified between normal and HPV positive cells"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - CD Genomics performed both the library preparation and sequencing. RNA was converted to cDNA before generating libraries using random primers","Nucleic Acid Extraction - Whole cell RNA was extracted using a Qiagen RNeasy mini kit and RIN values were verified to be >6","Sequencing - CD Genomics performed the sequencing. Generated cDNA was sequenced on an Ilumina HiSeq X with a read length of PE150.","Sample Collection - Primary cells (CIN612 and HFKs) were treated with versene to remove NIH-3T3-J2 feeder cells. All samples were then harvested and collected for RNA extraction."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Bowtie2 was used to remove rRNA from cleaned reads, and Hisat2 was used to map the subsequent reads to the hg38 reference genome. exomePeak2 was used to identify m6A peaks within each sample and differential m6A deposition across three replicates between HFK and CIN 612 cells."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina HiSeq X"],"study_type":["RIP-seq"],"species":["Homo sapiens"],"pubmed_authors":["Conor Templeton","Laimonis Laimins"],"additional_accession":[]},"is_claimable":false,"name":"m6A-RIP sequencing from primary human foreskin keratinocytes (HFKs) and HPV 31 positive cervical keratinocytes (CIN 612).","description":"Differentially m6A modified transcripts were identified between normal and HPV positive cells","dates":{"release":"2025-12-25T00:00:00Z","modification":"2026-05-27T14:48:21.548Z","creation":"2025-11-13T15:34:00.241Z"},"accession":"E-MTAB-16071","cross_references":{"ENA":["ERP184127"],"Biostudies":["E-MTAB-15997","E-MTAB-16046"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005310","EFO_0005518","EFO_0003816","EFO_0004184"]}}