biostudies-arrayexpresswenting xuHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16073We used three siRNAs to silence HSPA8 in MDA-MB-468 cells, and then used RNA-seq to compare HSPA8 knockdown cells with the controls.biostudies-arrayexpressNucleic Acid Extraction - 48-hours post-transfection, total RNA was extracted using TRIzol reagent (Ambion).Sample Collection - Cells transfected with siRNAs with Lipofectamin as the transfection reagent. Cells were cultured at 37°C in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and 100 IU/mL penicillin-streptomycin solution under 5% CO2.Sequencing - DNBSEQ-T7 system was used for 150 nt paired-end sequencing.Library Construction - VAHTS Universal V8 RNA-seq Library Prep Kit (Vazyme) was used to prepare strand-specific RNA-seq libraries.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - Raw sequencing reads containing more than 2-N bases were first discarded. Subsequently, the raw reads were trimmed of adaptors and low-quality bases using a FASTX-Toolkit (v.0.0.13; http://hannonlab.cshl.edu/fastx toolkit/). In addition, short reads of less than 16 nt were dropped to retain clean reads, which were subsequently aligned to the GRch38 genome by HISAT2 .Data Transformation - Uniquely mapped reads were used to calculate read number and paired-end fragments per kilobase of exon per million fragments mapped (FPKM) for each gene.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsDNBSEQ-T7RNA-seq of coding RNAHomo sapienswenting xufalseRNA-seq of HSPA8 knockdown in MDA-MB-468 cellsWe used three siRNAs to silence HSPA8 in MDA-MB-468 cells, and then used RNA-seq to compare HSPA8 knockdown cells with the controls.2025-11-13T00:00:00Z2026-05-30T17:00:18.4Z2025-11-06T13:02:38.963ZE-MTAB-16073ERP183681EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184