biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsJean-Baptiste DupontIllumina NovaSeq XRNA-seq of coding RNA from single cellsHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16077The experiment aimed to validate the gene network regulating cell fate decisions during the myogenic differentiation of iPS cells from a DMD patient. DMD iPSCs were transfected with specific siRNA at Day 7 of the differentiation protocol and collected either at Day 9 or Day 14. As a control, we used a non-specific siRNA. Non transfected DMD cells were also included, together with the healthy control cell line used in our previous experiments.biostudies-arrayexpressNucleic Acid Extraction - Nucleic acid extraction followed the split-pool barcoding protocol commercialized by Parse Biosciences under the name Evercode WT v2 kitSample Collection - Cells were collected from cultures of iPSCs in 2D culture plates. Dissociation was performed with Trypsin EDTA for 7min at 37°C, spinning for 5min at 1000g and fixation / permeabilisation using the Evercode Cell Fixation Kit (Parse Biosciences)Sequencing - Sequencing was performed on an illumina NovaSeq X plus with a 1.5B flowcell 200 cycles, using the following configuration: read 1: 66 cycles; index 1: 8 cycles; index 2: 8 cycles; read 2: 86 cycles.Library Construction - Library construction followed the split-pool barcoding protocol commercialized by Parse Biosciences under the name Evercode WT v2 kit. The initial samples included in the experiment were the following: DMD_None_J7; DMD_None_J9; DMD_None_J14; DMD_NC1_J9; DMD_NC1_J14; DMD_MIF_J9; DMD_MIF_J14; DMD_ENO1_J9; DMD_ENO1_J14; DMD_PRTG_J9; DMD_PRTG_J14; H_None_J7; H_None_J9; H_None_J14; H_NC1_J9; H_NC1_J14; H_ID1_J9; H_ID1_J14; H_PDGF_J9; H_PDGF_J14; H_PTN_J9; H_PTN_J14; AP_1; AP_2; AP_3; AP_4; AP_5; AP_6; AP_7; AP_8; AP_9; AP_10. After split-pool barcoding, the samples were pooled, the cells were lyzed and 10 different sublibraries were prepared for sequencing (AP-1 to AP-10). These sublibraries correspond to the 10 samples detailed in this submission. They each contain barcoded nucleic acids corresponding to the 32 cell samples detailed above.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesJean-Baptiste Dupontfalsesingle-cell RNA-Seq of human induced pluripotent stem cells from a healthy donor and a DMD patient at different time point during myogenic differentiationThe experiment aimed to validate the gene network regulating cell fate decisions during the myogenic differentiation of iPS cells from a DMD patient. DMD iPSCs were transfected with specific siRNA at Day 7 of the differentiation protocol and collected either at Day 9 or Day 14. As a control, we used a non-specific siRNA. Non transfected DMD cells were also included, together with the healthy control cell line used in our previous experiments.2026-06-01T00:00:00Z2026-06-01T01:00:57.322Z2025-11-13T13:33:50.528ZE-MTAB-16077ERP184200EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0004184