{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Naiqi Wang"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16107"],"description":["Weaning mice were fed with standard diet SF-NIH31 or SF-AIN93G (Specialty Feed, WA, Australia) for four weeks. Naive CD4+ or CD8+ cells were sorted for bulk RNA-sequencing. These two diets caused different ferroptosis sensitivity of T cells. The sequencing aims to elucidate if the diets alter ferroptosis-related gene expression."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Total RNA was extracted using the Rneasey Mini Kit (Qiagen), followed by quality assessment using the TapeStation system (Agilent).","Sample Collection - Mice splenocytes were collected. CD44-TCRb+ CD4+ or CD8+ naive T cells were sorted by flow cytometry.","Sequencing - Sequencing was performed by BGI (AU) on a DNBSEQ-G400 platform using following configuration: 29 bp Read1, 8 bp N5 Index, 8 bp N7 Index, 90 bp Read2","Library Construction - Library preparation was conducted by BGI Health (AU)"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - ChatGPT 说：  Raw RNA-seq reads were quality-trimmed to remove adapters and low-quality bases, then aligned to the mouse reference genome (mm10) using a standard short-read aligner to generate sorted BAM files for downstream analysis."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["BD FACSAria III","DNBSEQ-G400"],"study_type":["RNA-seq of coding RNA"],"species":["Mus musculus"],"pubmed_authors":["Naiqi Wang"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of mouse CD4+ and CD8+ cells after fed with SF-NIH31 or SF-AIN93G diet","description":"Weaning mice were fed with standard diet SF-NIH31 or SF-AIN93G (Specialty Feed, WA, Australia) for four weeks. Naive CD4+ or CD8+ cells were sorted for bulk RNA-sequencing. These two diets caused different ferroptosis sensitivity of T cells. The sequencing aims to elucidate if the diets alter ferroptosis-related gene expression.","dates":{"release":"2026-02-11T00:00:00Z","modification":"2026-05-27T13:44:25.459Z","creation":"2025-11-12T08:23:21.327Z"},"accession":"E-MTAB-16107","cross_references":{"ENA":["ERP184251"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184"]}}