biostudies-arrayexpressNaiqi WangMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16107Weaning mice were fed with standard diet SF-NIH31 or SF-AIN93G (Specialty Feed, WA, Australia) for four weeks. Naive CD4+ or CD8+ cells were sorted for bulk RNA-sequencing. These two diets caused different ferroptosis sensitivity of T cells. The sequencing aims to elucidate if the diets alter ferroptosis-related gene expression.biostudies-arrayexpressNucleic Acid Extraction - Total RNA was extracted using the Rneasey Mini Kit (Qiagen), followed by quality assessment using the TapeStation system (Agilent).Sample Collection - Mice splenocytes were collected. CD44-TCRb+ CD4+ or CD8+ naive T cells were sorted by flow cytometry.Sequencing - Sequencing was performed by BGI (AU) on a DNBSEQ-G400 platform using following configuration: 29 bp Read1, 8 bp N5 Index, 8 bp N7 Index, 90 bp Read2Library Construction - Library preparation was conducted by BGI Health (AU)OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - ChatGPT 说： Raw RNA-seq reads were quality-trimmed to remove adapters and low-quality bases, then aligned to the mouse reference genome (mm10) using a standard short-read aligner to generate sorted BAM files for downstream analysis.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsBD FACSAria IIIDNBSEQ-G400RNA-seq of coding RNAMus musculusNaiqi WangfalseRNA-seq of mouse CD4+ and CD8+ cells after fed with SF-NIH31 or SF-AIN93G dietWeaning mice were fed with standard diet SF-NIH31 or SF-AIN93G (Specialty Feed, WA, Australia) for four weeks. Naive CD4+ or CD8+ cells were sorted for bulk RNA-sequencing. These two diets caused different ferroptosis sensitivity of T cells. The sequencing aims to elucidate if the diets alter ferroptosis-related gene expression.2026-02-11T00:00:00Z2026-05-27T13:44:25.459Z2025-11-12T08:23:21.327ZE-MTAB-16107ERP184251EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184