biostudies-arrayexpressFrances MarksMycobacterium tuberculosishttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16127This experiment was done to look at the transcriptional profile of bacteria upon whiB2 transcriptional depletion. RNA-seq of an inducible whiB2 CRISPRi strain grown with 0 or 500 ng/mL anhydrotetracycline, which turns on the CRISPRi machinery. Samples were collected after 2 days of treatment.biostudies-arrayexpressLibrary Construction - Libraries were constructed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) using 150 ng of input RNA. Libraries were amplified using 8 cycles on the thermocycler. Post amplification libraries were size selected ay 250-450 bp in length using SparQ beads. Libraries were validated using Bioanalyzer high sensitivity DNA analysis.Nucleic Acid Extraction - RNA extraction was performed using Zymo RNA miniprep kitSequencing - Sequencing was done using a Illumina NovaSeq X PlusSample Collection - Bacteria were mixed with 5M GTC, pelleted, and resuspended in Trizol.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - Trimmed a filtered sequencing reads were mapped to the H37Rv reference genome (GenBank AL123456.3; RefSeq NC_000962.3; GCF_000195955.2; Genome assembly ASM19595v2)Data Transformation - Raw read counts were done using featureCounts and normalized read counts were done using DESeq2MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XRNA-seq of coding RNAMycobacterium tuberculosisFrances MarksfalseRNA-seq of an inducible whiB2 CRISPRi strain grown with 0 or 500 ng/mL anhydrotetracycline, which turns on the CRISPRi machineryThis experiment was done to look at the transcriptional profile of bacteria upon whiB2 transcriptional depletion. RNA-seq of an inducible whiB2 CRISPRi strain grown with 0 or 500 ng/mL anhydrotetracycline, which turns on the CRISPRi machinery. Samples were collected after 2 days of treatment.2026-08-06T00:00:00Z2026-08-06T21:35:42.924Z2025-11-14T13:19:52.251ZE-MTAB-16127ERP184448EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184