biostudies-arrayexpressFrances MarksMycobacterium tuberculosishttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16129In vitro DNA affinity purification sequencing of recombinant WhiA incubated with sheard genomic DNA.biostudies-arrayexpressSample Collection - 200 nM of recombinant His-tagged WhiA was incubated with 4 µg of sheared mycobacterium tuberculosis genomic DNANucleic Acid Extraction - Protein-DNA mixtures were incubated with Talon metal affinity resin (Takara Bio 635501) to pulldown His-tagged WhiA and any DNA bound by the protein. DNA was eluted by boiling protein in SDS-containing buffer. DNA was purified using QIAquick PCR Purification Kit (Qiagen 28104)Library Construction - Libraries were constructed using NEBNext Ultra II Directional DNA Library Prep Kit for Illumina (New England Biolabs) using 500 pg of input DNA. Libraries were amplified using 14 cycles on the thermocycler. Post amplification libraries were size selected ay 250-450 bp in length using SparQ beads. Libraries were validated using Bioanalyzer high sensitivity DNA analysis.Sequencing - Samples were sequenced using Illumina NovaSeq 6000OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - Trimmed a filtered sequencing reads were mapped to the H37Rv reference genome (GenBank AL123456.3; RefSeq NC_000962.3; GCF_000195955.2; Genome assembly ASM19595v2)Data Transformation - BAM files were converted to bigWig files using the Bioconductor package rtracklayer. Peak calling was performed using MACS2MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000ChIP-seqMycobacterium tuberculosisFrances MarksfalseIDAP-seq of recombinant WhiA incubated with mycobacterium tuberculosis genomic DNAIn vitro DNA affinity purification sequencing of recombinant WhiA incubated with sheard genomic DNA.2026-08-06T00:00:00Z2026-08-06T21:35:12.735Z2025-11-14T13:31:30.394ZE-MTAB-16129ERP184450EFO_0002944EFO_0004170EFO_0002692EFO_0004917EFO_0005518EFO_0003816EFO_0004184