{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Aniuska Becerra"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16144"],"description":["Assessment of the functional differences of HHV-6B-specific and HCMV-specific CD4 T cells at RNA level. The peripheral blood mononuclear cells samples were collected from normal subjects expressing HLA-DRB1*03:01. The HHV-6B-specific or HCMV-specific T cells were expanded in vitro using known HLA-DRB1*03:01 epitopes derived from HHV-6B (6 epitopes) or HCMV (1 epitope) and isolated after 12-15 days of culture using the corresponding HLA-DRB1*03:01 tetramers."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - Libraries were pooled and sequenced in the Illumina HiSeq4000 System, single-read, 100 cycles.","Sample Collection - Epitope-specific CD4 T cells were isolated from PBMC cultured in vitro in presence of epitope peptides (10 ug/mL) for ~12-15 days, with IL2 addition to the medium at days 4 and 8. Isolation was performed using DRB1*03:01 tetramers carrying the epitope peptides and flow cytometry-based cell sorting.","Nucleic Acid Extraction - Total RNA was extracted from tetramer-sorted cells using RNeasy kit (Qiagen).","Library Construction - Libraries were prepared from 250 ng of RNA with RQN>7.2, using the Illumina TruSeq v2 platform and following manufacturer's instructions."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Demultiplexed raw data was processed using DoplhinNext RNA-Seq pipeline (1.0.0) (UMassMed Server Vpipe)."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina HiSeq 4000"],"study_type":["RNA-seq of total RNA"],"species":["Homo sapiens"],"pubmed_authors":["Aniuska Becerra"],"additional_accession":[]},"is_claimable":false,"name":"Differential gene expression analysis of in vitro expanded CD4 T cells responding to HHV-6B and HCMV epitopes","description":"Assessment of the functional differences of HHV-6B-specific and HCMV-specific CD4 T cells at RNA level. The peripheral blood mononuclear cells samples were collected from normal subjects expressing HLA-DRB1*03:01. The HHV-6B-specific or HCMV-specific T cells were expanded in vitro using known HLA-DRB1*03:01 epitopes derived from HHV-6B (6 epitopes) or HCMV (1 epitope) and isolated after 12-15 days of culture using the corresponding HLA-DRB1*03:01 tetramers.","dates":{"release":"2025-11-20T00:00:00Z","modification":"2025-11-20T18:34:43.044Z","creation":"2025-11-20T12:06:34.008Z"},"accession":"E-MTAB-16144","cross_references":{"ENA":["ERP185408"],"EFO":["EFO_0002944","EFO_0004170","EFO_0009653","EFO_0005518","EFO_0003816","EFO_0004184"]}}