biostudies-arrayexpressAniuska BecerraHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16144Assessment of the functional differences of HHV-6B-specific and HCMV-specific CD4 T cells at RNA level. The peripheral blood mononuclear cells samples were collected from normal subjects expressing HLA-DRB1*03:01. The HHV-6B-specific or HCMV-specific T cells were expanded in vitro using known HLA-DRB1*03:01 epitopes derived from HHV-6B (6 epitopes) or HCMV (1 epitope) and isolated after 12-15 days of culture using the corresponding HLA-DRB1*03:01 tetramers.biostudies-arrayexpressSequencing - Libraries were pooled and sequenced in the Illumina HiSeq4000 System, single-read, 100 cycles.Sample Collection - Epitope-specific CD4 T cells were isolated from PBMC cultured in vitro in presence of epitope peptides (10 ug/mL) for ~12-15 days, with IL2 addition to the medium at days 4 and 8. Isolation was performed using DRB1*03:01 tetramers carrying the epitope peptides and flow cytometry-based cell sorting.Nucleic Acid Extraction - Total RNA was extracted from tetramer-sorted cells using RNeasy kit (Qiagen).Library Construction - Libraries were prepared from 250 ng of RNA with RQN>7.2, using the Illumina TruSeq v2 platform and following manufacturer's instructions.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Demultiplexed raw data was processed using DoplhinNext RNA-Seq pipeline (1.0.0) (UMassMed Server Vpipe).MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina HiSeq 4000RNA-seq of total RNAHomo sapiensAniuska BecerrafalseDifferential gene expression analysis of in vitro expanded CD4 T cells responding to HHV-6B and HCMV epitopesAssessment of the functional differences of HHV-6B-specific and HCMV-specific CD4 T cells at RNA level. The peripheral blood mononuclear cells samples were collected from normal subjects expressing HLA-DRB1*03:01. The HHV-6B-specific or HCMV-specific T cells were expanded in vitro using known HLA-DRB1*03:01 epitopes derived from HHV-6B (6 epitopes) or HCMV (1 epitope) and isolated after 12-15 days of culture using the corresponding HLA-DRB1*03:01 tetramers.2025-11-20T00:00:00Z2025-11-20T18:34:43.044Z2025-11-20T12:06:34.008ZE-MTAB-16144ERP185408EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0003816EFO_0004184