biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsNada Salehtranscription profiling by arrayHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16146This dataset includes RT-qPCR fold-change expression data for apoptosis-, proliferation-, and inflammation-related genes (BAX, BCL-2, CASPASE-3, P53, Ki-67, and IL-6) in two oral cancer cell lines (HEP-2 and HN-9) treated with free Captopril and Captopril-Montmorillonite nanoparticles (CP-MMT). The study demonstrates the enhanced pro-apoptotic and oxidative stress effects of Captopril nanoparticles compared to free Captopril.biostudies-arrayexpressGrowth Protocol - Cells were maintained in DMEM supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin at 37 °C and 5% CO₂ in a humidified incubator.Scaning - Ct values were exported from the StepOnePlus software and analyzed in Microsoft Excel. Fold changes were computed relative to untreated controls to generate normalized expression data for each target gene.Hybridization - Real-time PCR amplification was carried out using SYBR Green Master Mix on a StepOnePlus™ Real-Time PCR System (Applied Biosystems). Relative mRNA expression levels were calculated using the 2^-ΔΔCt method, with β-actin as the internal control.Sample Collection - Oral squamous carcinoma cell lines (HEP-2 and HN-9) were cultured under standard conditions (37 °C, 5% CO₂) in DMEM supplemented with 10% FBS and antibiotics. Cells were seeded in 6-well plates and treated according to the experimental design.Labeling - cDNA synthesis was performed using a high-capacity cDNA reverse transcription kit (Applied Biosystems) following the manufacturer’s protocol.Sample Treatment - Cells were treated with free Captopril or Captopril-Montmorillonite (CP-MMT) nanoparticles for 48 hours. Untreated cells served as controls.Nucleic Acid Extraction - Total RNA was extracted from treated and control cells using TRIzol reagent according to the manufacturer’s instructions. RNA purity and concentration were determined using a NanoDrop spectrophotometer (260/280 nm ratio).MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsNada SalehData Transformation - Relative gene expression values were calculated using the comparative Ct (2^-ΔΔCt) method. Data were normalized to β-actin and presented as fold change relative to control.falseGene Expression Profiling of Oral Cancer Cell Lines Treated with Captopril and Captopril-Montmorillonite Nanoparticles (CP-MMT)This dataset includes RT-qPCR fold-change expression data for apoptosis-, proliferation-, and inflammation-related genes (BAX, BCL-2, CASPASE-3, P53, Ki-67, and IL-6) in two oral cancer cell lines (HEP-2 and HN-9) treated with free Captopril and Captopril-Montmorillonite nanoparticles (CP-MMT). The study demonstrates the enhanced pro-apoptotic and oxidative stress effects of Captopril nanoparticles compared to free Captopril.2025-12-08T00:00:00Z2025-12-08T02:01:32.968Z2025-11-16T13:07:32.017ZE-MTAB-16146EFO_0002768EFO_0002944EFO_0003814EFO_0003813EFO_0003789EFO_0005518EFO_0003816EFO_0003815EFO_0003969