biostudies-arrayexpressHaoqing HuHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16152Engineered SOX17 (eSOX17) factors were used to replace Yamanaka factor SOX2 in induced expanded potential stem cell (EPSC) reprogramming. eSOX17 achieved more efficient reprogramming under both feeder and feeder-free conditions. We have compared the iEPSC cell lines generated during the reprogramming with the embryo-derived EPSC control and found a similar gene expression pattern.biostudies-arrayexpressLibrary Construction - Library was prepared by AZENTA using Poly-A selectionSample Collection - Total RNA was extracted using Trizol (Invitrogen, #15596018) and more than 2 μg total RNA of each sample was submitted to AZENTA (Suzhou, China) for 150 bp paired-end RNA-seqNucleic Acid Extraction - Total RNA was extracted using Trizol (Invitrogen, #15596018)Sequencing - Sequencing was performed on the Illumina Novaseq platform, in a 2x150bp paired-end (PE) configuration, with ~6.0 Gb PF data per sampleOrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Raw RNA-seq reads in fastq format were aligned to the hg38 transcriptome using RSEM and bowtie 2.0. Alignment results at the gene levels for multiple samples were merged and annotated using glbase3. Read counts per gene were calculated using RSEM and normalized to GC content using EDAseq. GC-normalized read counts ≥ 20 in at least two samples were kept. PCA was performed in R (stats::prcomp) on log2-transformed (rounded counts + 1), centered data; results were visualized with ggplot2/ggrepel.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsNot applicableIllumina NovaSeq 6000RNA-seq of coding RNAHomo sapiensHaoqing HufalseEnhancing reprogramming towards human expanded pluripotencyEngineered SOX17 (eSOX17) factors were used to replace Yamanaka factor SOX2 in induced expanded potential stem cell (EPSC) reprogramming. eSOX17 achieved more efficient reprogramming under both feeder and feeder-free conditions. We have compared the iEPSC cell lines generated during the reprogramming with the embryo-derived EPSC control and found a similar gene expression pattern.2026-03-12T00:00:00Z2026-03-17T14:46:17.095Z2025-11-17T11:42:22.084ZE-MTAB-16152ERP185158EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184