biostudies-arrayexpressguosheng WangRattus norvegicushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16153We constructed a rat model of femoral osteomyelitis by injecting a mixture of Staphylococcus aureus suspension and LPS. Subsequently, we performed RNA-seq to compare the osteomyelitic femur with the normal femur group.biostudies-arrayexpressSample Collection - In this study, five femoral tissue samples were collected from the chronic osteomyelitis model group and the control group each, resulting in a total of ten samples.Library Construction - For each sample, 500 ng of total RNA was used for cDNA synthesis via a strand-switching reverse transcription approach. The resulting cDNA was then amplified by PCR, followed by barcoding with the Native Barcode kit (SQK-NBD114.96) and purification using Ampure XP Beads. Finally, 300 fmol of the resulting library was loaded onto a PromethION flow cell (vR10.4.4) for sequencing.Nucleic Acid Extraction - Total RNA extraction using Total RNA extraction Kit (DP431, TIANGEN, China). The quality and quantity of the purified RNA were determined by measuring the absorbance at 260nm/280nm(A260/A280) using Ultrafine spectrophotometer (N50 touch, IMPLEN, Germany). The integrity of RNA was further verified by 1.0% agarose gel electrophoresisSequencing - We used the Oxford nanopore technology (ONT) to sequence from AS and HCOrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - To evaluate the accuracy of abundance estimation with transcript and gene resolution, we used the quasi-mapping mode of Salmon(v1.7.0) [17] to estimate the TPM for each transcript and gene in each of the sample data.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsPromethIONRNA-seq of coding RNARattus norvegicusguosheng WangfalseRNA-seq of rat tissues with chronic osteomyelitisWe constructed a rat model of femoral osteomyelitis by injecting a mixture of Staphylococcus aureus suspension and LPS. Subsequently, we performed RNA-seq to compare the osteomyelitic femur with the normal femur group.2025-12-10T00:00:00Z2026-05-30T17:25:19.459Z2025-11-11T14:59:52.041ZE-MTAB-16153ERP184213EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184