biostudies-arrayexpressRyusuke SawadaMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16154To enhance local drug delivery and therapeutic efficacy, we developed a novel synthetic protein by fusing a collagen-binding domain (CBD) to C-type natriuretic peptide (CNP), termed CBD-CNP. CBD-CNP localized to collagen-rich tissues and promoted bone elongation.biostudies-arrayexpressSample Collection - Three fresh frozen tibiae per group were OCT embedded, and 10μm frozen sections were cut with cryostat.Library Construction - Spatial transcriptome sequencing libraries were prepared with the Visium Spatial Gene Expression Slide & Reagent Kit (10x Genomics) according to the manufacturer's instructions.Nucleic Acid Extraction - Nucleic acid was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions.Sample Treatment - The tibiae were divided into two groups (n = 3 per group): one treated with FGF2 alone and the other with FGF2 plus CBD-CNP group. In the FGF2 group, 100 ng/mL FGF2 was administered unilaterally. In the CBD-CNP group, one side received 100 ng/mL FGF2, whereas the opposite site was treated with 100 ng/mL FGF2 and 0.2 µM CBD-CNP for four days with daily medium changes.Growth Protocol - The tibiae from embryonic day 16.5 mice were cultured in a 24-well plate.Sequencing - Libraries were profiled with the High Sensitivity D5000 ScreenTape assay (Agilent Technologies) and sequenced on a DNBSEQ-G400 (MGI), with a minimum of 50,000 read pairs per spot.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - Sequencing data were aligned to the mm10-2020-A reference genome to gene expression count using default settings of Space Ranger v.2.0.1 (10x Genomics).Data Transformation - Raw sequencing data were processed using Space Ranger (v2.0.1). Reads were aligned to mm10-2020-A 10x reference.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsDNBSEQ-G400spatial transcriptomics by high-throughput sequencingMus musculusCollagen-binding C-type natriuretic peptide enhances chondrogenesis and osteogenesisKenta HiraiRyusuke SawadaKenta Hirai, Kenta Sawamura, Ryusaku Esaki, Ryusuke Sawada, Yuka Okusha, Eriko Aoyama, Hiroki Saito, Kentaro Uchida, Takehiko Mima, Satoshi Kubota, Hirokazu Tsukahara1, Shiro Imagama, Masaki Matsushita, Osamu Matsushita, Yasuyuki HosonofalseCollagen-binding C-type natriuretic peptide enhances chondrogenesis and osteogenesisTo enhance local drug delivery and therapeutic efficacy, we developed a novel synthetic protein by fusing a collagen-binding domain (CBD) to C-type natriuretic peptide (CNP), termed CBD-CNP. CBD-CNP localized to collagen-rich tissues and promoted bone elongation.2025-12-05T00:00:00Z2026-05-27T16:34:26.164Z2025-11-17T11:56:04.749ZE-MTAB-16154ERP185162EFO_0002944EFO_0004170EFO_0003789EFO_0030005EFO_0004917EFO_0005518EFO_0003816EFO_0004184EFO_0003969