{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["wenjun yin"],"instrument_platform":["DNBSEQ-T7"],"study_type":["RNA-seq of coding RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16163"],"description":["C57BL/6J mice were subjected to 30 Gy liver irradiation (5 Gy × 6 fractions). METTL14 overexpression was achieved via AAV-METTL14 tail-vein injection. Liver tissues were collected for RNA-seq analysis to identify differentially expressed genes (DEGs) related to cell death pathways."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Cells were cultured at 37 °C in DMEM supplemented with 10 % FBS and 1 % penicillin-streptomycin under 5 % CO₂.","Library Construction - Paired-end 150 bp sequencing was performed on Illumina NovaSeq 6000 platform according to manufacturer instructions.","Sequencing - Cells were harvested at 80 % confluence, washed twice with cold PBS, and snap-frozen in liquid nitrogen.","Sample Collection - PolyA mRNA was enriched using oligo-dT beads, fragmented, and converted to cDNA with TruSeq Stranded mRNA Library Prep Kit."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["wenjun yin"],"additional_accession":[]},"is_claimable":false,"name":"The identification of genes related to METTL14 overexpression in radiation-induced hepatocyte death based on bioinformatics analysis","description":"C57BL/6J mice were subjected to 30 Gy liver irradiation (5 Gy × 6 fractions). METTL14 overexpression was achieved via AAV-METTL14 tail-vein injection. Liver tissues were collected for RNA-seq analysis to identify differentially expressed genes (DEGs) related to cell death pathways.","dates":{"release":"2026-05-04T00:00:00Z","modification":"2026-05-04T08:32:58.359Z","creation":"2025-11-17T13:59:35.993Z"},"accession":"E-MTAB-16163","cross_references":{"ENA":["ERP185173"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}