biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicswenjun yinDNBSEQ-T7RNA-seq of coding RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16163C57BL/6J mice were subjected to 30 Gy liver irradiation (5 Gy × 6 fractions). METTL14 overexpression was achieved via AAV-METTL14 tail-vein injection. Liver tissues were collected for RNA-seq analysis to identify differentially expressed genes (DEGs) related to cell death pathways.biostudies-arrayexpressNucleic Acid Extraction - Cells were cultured at 37 °C in DMEM supplemented with 10 % FBS and 1 % penicillin-streptomycin under 5 % CO₂.Library Construction - Paired-end 150 bp sequencing was performed on Illumina NovaSeq 6000 platform according to manufacturer instructions.Sequencing - Cells were harvested at 80 % confluence, washed twice with cold PBS, and snap-frozen in liquid nitrogen.Sample Collection - PolyA mRNA was enriched using oligo-dT beads, fragmented, and converted to cDNA with TruSeq Stranded mRNA Library Prep Kit.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB Fileswenjun yinfalseThe identification of genes related to METTL14 overexpression in radiation-induced hepatocyte death based on bioinformatics analysisC57BL/6J mice were subjected to 30 Gy liver irradiation (5 Gy × 6 fractions). METTL14 overexpression was achieved via AAV-METTL14 tail-vein injection. Liver tissues were collected for RNA-seq analysis to identify differentially expressed genes (DEGs) related to cell death pathways.2026-05-04T00:00:00Z2026-05-04T08:32:58.359Z2025-11-17T13:59:35.993ZE-MTAB-16163ERP185173EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184