biostudies-arrayexpressFrances MarksMycobacterium tuberculosishttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16173ChIP-seq of mycobacterium tuberculosis containing an N-terminal FLAG-tagged WhiA allelebiostudies-arrayexpressSample Collection - Cells were fixed with 1% paraformaldehyde for 30 min and the reaction was quenched by treating with 250 mM glycine.Nucleic Acid Extraction - Cells were bead beated, cell lysates were collected, and chromatin was sheared using a Covaris M220Sequencing - Libraries were sequenced using Illumina NovaSeq 6000Growth Protocol - 7H9, 0.2% glycerol 10% OADC, 0.05% tyloxapol, 25 µg/mL kanamycin, 50 ug/mL hygromycin. Cells were cultured at 37C with 5% CO2Library Construction - Libraries were constructed using NEBNext Ultra II Directional DNA Library Prep Kit for Illumina (New England Biolabs) using 500 pg of input DNA. Libraries were amplified using 14 cycles on the thermocycler. Post amplification libraries were size selected ay 250-450 bp in length using SparQ beads. Libraries were validated using Bioanalyzer high sensitivity DNA analysis.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - BAM files were converted to bigWig files using the Bioconductor package rtracklayer. Peak calling was performed using MACS.Sequence Alignment - Trimmed a filtered sequencing reads were mapped to the H37Rv reference genome (GenBank AL123456.3; RefSeq NC_000962.3; GCF_000195955.2; Genome assembly ASM19595v2). BAM files were converted to bigWig files using the Bioconductor package rtracklayer. Peak calling was performed using MACS2 .MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000ChIP-seqMycobacterium tuberculosisFrances MarksfalseChIP-seq of N-terminal FLAG tagged WhiAChIP-seq of mycobacterium tuberculosis containing an N-terminal FLAG-tagged WhiA allele2026-08-06T00:00:00Z2026-08-06T21:34:38.767Z2025-11-18T10:18:09.872ZE-MTAB-16173ERP185210EFO_0002944EFO_0004170EFO_0003789EFO_0002692EFO_0004917EFO_0005518EFO_0003816EFO_0004184