biostudies-arrayexpressAniuska BecerraHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16174Assessment of the gene expression differences between HHV-6B-specific and HCMV-specific CD4 T cells at single-cell level. The peripheral blood mononuclear cells samples were collected from normal subjects expressing HLA-DRB1*03:01. The HHV-6B-specific or HCMV-specific T cells were expanded in vitro using known HLA-DRB1*03:01 epitopes derived from HHV-6B (6 epitopes) or HCMV (1 epitope) and isolated after 12-15 days of culture using the corresponding HLA-DRB1*03:01 tetramers.biostudies-arrayexpressSequencing - Libraries were pooled and sequenced in the Illumina HiSeq4000 System, paired-reads, 26x98 cycles.Sample Collection - Epitope-specific CD4 T cells were isolated from PBMC cultured in vitro in presence of epitope peptides (10 ug/mL) for ~12-15 days, with IL2 addition to the medium at days 4 and 8. Isolation was performed using DRB1*03:01 tetramers carrying the epitope peptides and flow cytometry-based cell sorting.Library Construction - Library was prepared using the 10X Genomics Chromium Single Cell 5’ Library Construction Kit v1.1, following manufacturer's instructions.Nucleic Acid Extraction - RNA from single cells were obtained using the 10X Genomics Chromium Next GEM Single Cell 5’ Library & Gel Bead Kit v1.1, following manufacturer's instructions.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Data processing, counting, and aggregation were done using the 10X Genomics Cell Ranger 3.1.0 and Loupe Cell Browser 3.1.1 pipelines.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina HiSeq 4000RNA-seq of coding RNA from single cellsHomo sapiensAniuska BecerrafalseSingle-cell gene expression analysis of in vitro expanded CD4 T cells responding to HHV-6B and HCMV epitopesAssessment of the gene expression differences between HHV-6B-specific and HCMV-specific CD4 T cells at single-cell level. The peripheral blood mononuclear cells samples were collected from normal subjects expressing HLA-DRB1*03:01. The HHV-6B-specific or HCMV-specific T cells were expanded in vitro using known HLA-DRB1*03:01 epitopes derived from HHV-6B (6 epitopes) or HCMV (1 epitope) and isolated after 12-15 days of culture using the corresponding HLA-DRB1*03:01 tetramers.2025-11-21T00:00:00Z2026-05-27T16:00:22.607Z2025-11-21T15:39:13.355ZE-MTAB-16174ERP185489E-MTAB-16144EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0003816EFO_0004184