biostudies-arrayexpressFrances MarksMycobacterium tuberculosishttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16185ChIP-seq of Mycobacterium tuberculosis strain with a C-terminal FLAG-tagged WhiA allele compared to a strain carrying a FLAG attached to no protein (empty)biostudies-arrayexpressNucleic Acid Extraction - Cells were bead beated, cell lysates were collected, and chromatin was sheared using a Covaris M220Sample Collection - Cells were fixed with 1% paraformaldehyde for 30 min and the reaction was quenched by treating with 250 mM glycineGrowth Protocol - 7H9, 0.2% glycerol 10% OADC, 0.05% tyloxapol, 25 µg/mL kanamycin, 50 ug/mL hygromycin. Cells were cultured at 37C with 5% CO2Library Construction - Libraries were constructed using NEBNext Ultra II Directional DNA Library Prep Kit for Illumina (New England Biolabs) using 500 pg of input DNA. Libraries were amplified using 14 cycles on the thermocycler. Post amplification libraries were size selected ay 250-450 bp in length using SparQ beads. Libraries were validated using Bioanalyzer high sensitivity DNA analysis.Sequencing - Samples were sequenced using an Illumina NovaSeq X PlusOrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - Trimmed a filtered sequencing reads were mapped to the H37Rv reference genome (GenBank AL123456.3; RefSeq NC_000962.3; GCF_000195955.2; Genome assembly ASM19595v2)Data Transformation - BAM files were converted to bigWig files using the Bioconductor package rtracklayer. Peak calling was performed using MACS2MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XRNA-seq of coding RNAMycobacterium tuberculosisFrances MarksfalseChIP-seq of C-terminal FLAG-tagged WhiAChIP-seq of Mycobacterium tuberculosis strain with a C-terminal FLAG-tagged WhiA allele compared to a strain carrying a FLAG attached to no protein (empty)2026-08-06T00:00:00Z2026-08-06T21:33:58.867Z2025-11-19T10:53:53.413ZE-MTAB-16185ERP185314EFO_0002944EFO_0004170EFO_0003789EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184