biostudies-arrayexpressYa Chun YuMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16191The aim of this study is to evaluate the changes in the chromatin accessibility landscape of BMDMs from WT and SLC1A5_var transgenic mice after low-dose LPS training. We compared the data of ATAC-seq performed on nuclei extracted from naive and trained BMDM from both genotypes. Inter-group comparisons revealed differentially accessible regions to occur between traind WT and Tg BMDMs. Since SLC1A5_var function as unique mitochondrial glutamine transporter, the results indicate that altered glutaminolysis could impact chromatin accessbility.biostudies-arrayexpressNucleic Acid Extraction - Approximately 1 × 105 fresh cells were washed twice with ice-cold PBS and resuspended in 100 µl of lysis buffer for 10 min to extract the nuclei. The nuclei were then incubated with Tagmentation Master Mix for 30 min at 37℃.Library Construction - PCR was performed to amplify the library, and then, SPRI beads (Beckman Coulter) were used to clear the contaminating primer dimers. Library construction was performed with the ATAC-Seq Library Prep Kit for Illumina (Active Motif).Sample Collection - BMDM form both of WT and SLC1A5_var were trained with PBS or LPS overnight. Cells were washed and rested until day 6 and harvested for nuclei extraction.Sequencing - A library quantity check was performed with TapeStation HSD5000 Screen Tape (Agilent Technologies). All libraries were sequenced on the NovaSeq XP system with 150-bp paired-end reads (Illumina).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XATAC-seqMus musculusMitochondrial Glutaminolysis Governs the Epigenetic Training of Macrophages for Antitumor Immunity.Ya Chun Yu, Yulseung Sung, Seonghun Lim, Jeoung Ah Kwon, Kuglae Kim, Do Sik Min, Hee Chan Yoo, Jung Min HanJung Min HanYa Chun YufalseATAC-seq analysis of LPS-trained bone marrow derived macrophages (BMDMs) from WT and SLC1A5_var transgenic mice.The aim of this study is to evaluate the changes in the chromatin accessibility landscape of BMDMs from WT and SLC1A5_var transgenic mice after low-dose LPS training. We compared the data of ATAC-seq performed on nuclei extracted from naive and trained BMDM from both genotypes. Inter-group comparisons revealed differentially accessible regions to occur between traind WT and Tg BMDMs. Since SLC1A5_var function as unique mitochondrial glutamine transporter, the results indicate that altered glutaminolysis could impact chromatin accessbility.2026-06-24T00:00:00Z2026-06-24T03:04:20.553Z2025-11-19T11:15:35.673ZE-MTAB-16191ERP191607EFO_0002944EFO_0007045EFO_0004170EFO_0005518EFO_0004184