biostudies-arrayexpressYa Chun YuMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16196The aim of this study is to profile the distribution of the active histone modification H3K4me3 in BMDMs from WT and SLC1A5_var transgenic mice after low-dose LPS training. We compared the data of ChIP-seq performed on fragmented chromatin immunoprecipitated with anti-H3K4me3 antibody in naive and trained BMDM from both genotypes. Inter-group comparisons revealed increased differentially enriched peaks near promoters of pro-inflammatory genes of trained WT BMDMs, where as this changes were blunted in Tg BMDMs. Since SLC1A5_var function as unique mitochondrial glutamine transporter, the results indicate that altered glutaminolysis could impact epigenetic regulation under LPS-training,biostudies-arrayexpressLibrary Construction - Purified DNA was subjected to library construction using the NEBNext® UltraTM DNA Library Prep Kit for Illumina (New England Biolabs). In brief, the chipped DNA was ligated with adaptors for PCR with index primers for multiplexing sequencing.Sample Collection - BMDM form both of WT and SLC1A5_var were trained with PBS or LPS overnight. Cells were washed and rested until day 6 and harvested.Nucleic Acid Extraction - Approximately 5 × 106 BMDMs were fixed, and the nuclei were collected, after which the chromatin was isolated and immunoprecipitated using an anti-histone H3 (trimethylated K4) antibody (Abcam) and a high-sensitivity ChIP kit (Abcam) according to the manufacturer’s instructions.Sequencing - High-throughput sequencing was performed as paired-end 150-bp sequencing using NovaSeq X Plus (Illumina).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XChIP-seqMus musculusMitochondrial Glutaminolysis Governs the Epigenetic Training of Macrophages for Antitumor Immunity.Ya Chun Yu, Yulseung Sung, Seonghun Lim, Jeoung Ah Kwon, Kuglae Kim, Do Sik Min, Hee Chan Yoo, Jung Min HanJung Min HanYa Chun YufalseChIP-seq analysis of LPS trained BMDMs reveals remodeling of Histone 3 Lysine 4 tri-methylation (H3K4me3)The aim of this study is to profile the distribution of the active histone modification H3K4me3 in BMDMs from WT and SLC1A5_var transgenic mice after low-dose LPS training. We compared the data of ChIP-seq performed on fragmented chromatin immunoprecipitated with anti-H3K4me3 antibody in naive and trained BMDM from both genotypes. Inter-group comparisons revealed increased differentially enriched peaks near promoters of pro-inflammatory genes of trained WT BMDMs, where as this changes were blunted in Tg BMDMs. Since SLC1A5_var function as unique mitochondrial glutamine transporter, the results indicate that altered glutaminolysis could impact epigenetic regulation under LPS-training,2026-06-24T00:00:00Z2026-06-24T03:04:00.41Z2025-11-19T11:38:57.708ZE-MTAB-16196ERP191643EFO_0002944EFO_0004170EFO_0002692EFO_0005518EFO_0004184