biostudies-arrayexpressFrances MarksMycobacterium tuberculosishttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16198ChIP-seq of mycobacterium tuberculosis expressing a 3xFLAG-tagged N-terminal WhiB2 compared with a strain carrying an empty FLAG (not attached to a protein)biostudies-arrayexpressNucleic Acid Extraction - Cells were bead beated, cell lysates were collected, and chromatin was sheared using a Covaris M220Sample Collection - Cells were fixed with 1% paraformaldehyde for 30 min and the reaction was quenched by treating with 250 mM glycineLibrary Construction - Libraries were constructed using NEBNext Ultra II Directional DNA Library Prep Kit for Illumina (New England Biolabs) using 500 pg of input DNA. Libraries were amplified using 14 cycles on the thermocycler. Post amplification libraries were size selected ay 250-450 bp in length using SparQ beads. Libraries were validated using Bioanalyzer high sensitivity DNA analysis.Sequencing - Samples were sequenced using an Illumina NovaSeq X PlusOrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - BAM files were converted to bigWig files using the Bioconductor package rtracklayer. Peak calling was performed using MACS2 .Sequence Alignment - Trimmed a filtered sequencing reads were mapped to the H37Rv reference genome (GenBank AL123456.3; RefSeq NC_000962.3; GCF_000195955.2; Genome assembly ASM19595v2)MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XChIP-seqMycobacterium tuberculosisFrances MarksfalseChIP-seq of 3xFLAG-WhiB2ChIP-seq of mycobacterium tuberculosis expressing a 3xFLAG-tagged N-terminal WhiB2 compared with a strain carrying an empty FLAG (not attached to a protein)2026-08-06T00:00:00Z2026-08-06T21:32:57.964Z2025-11-19T12:02:03.128ZE-MTAB-16198ERP185325EFO_0002944EFO_0004170EFO_0002692EFO_0004917EFO_0005518EFO_0003816EFO_0004184