{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Max Thorwald"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16199"],"description":["C57BL/6 Mouse cortex samples at 2-months of age, both sexes, exposed to one acute 5-hour dose of 100ug/m3 of air pollution from diesel exhaust particles or dust from the World Trade Center collapse. C57BL/6 female mouse cortex exposed to one acute 4-hour dose of 500ug/m3 of woodsmoke."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - 20 mg of brain tissue was homogenized in TRIzol reagent using a BeadBug Benchtop Homogenizer. Brain tissue was suspended in 1 mL of TRIzol and homogenized for 6 rounds of 10 seconds homogenization and 60-second holds between each round. Following homogenization, 300 µL of chloroform was added, and samples were centrifuged with heavy gel phase-lock tubes (VWR, 10847-802) to separate the aqueous phase.","Library Construction - Library preparation (mRNA library, poly A enrichment) was performed by Novogene.","Nucleic Acid Extraction - RNA purification kit (Quantabio, Extracta Plus, Cat# 95214-050) was used following the manufacturer’s protocol. RNA concentration and integrity were assessed with a Qubit 4.0 Fluorometer and Agilent Bioanalyzer prior to sequencing.","Sequencing - RNA sequencing (NovoSeq PE150. 6G raw data per sample) was performed by Novogene."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Max Thorwald"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptomic responses to diesel exhaust particles or World trade center dust","description":"C57BL/6 Mouse cortex samples at 2-months of age, both sexes, exposed to one acute 5-hour dose of 100ug/m3 of air pollution from diesel exhaust particles or dust from the World Trade Center collapse. C57BL/6 female mouse cortex exposed to one acute 4-hour dose of 500ug/m3 of woodsmoke.","dates":{"release":"2025-12-22T00:00:00Z","modification":"2025-12-22T02:01:18.926Z","creation":"2025-11-19T13:32:19.781Z"},"accession":"E-MTAB-16199","cross_references":{"ENA":["ERP185330"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}