biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsMax ThorwaldIllumina NovaSeq 6000RNA-seq of coding RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16199C57BL/6 Mouse cortex samples at 2-months of age, both sexes, exposed to one acute 5-hour dose of 100ug/m3 of air pollution from diesel exhaust particles or dust from the World Trade Center collapse. C57BL/6 female mouse cortex exposed to one acute 4-hour dose of 500ug/m3 of woodsmoke.biostudies-arrayexpressSample Collection - 20 mg of brain tissue was homogenized in TRIzol reagent using a BeadBug Benchtop Homogenizer. Brain tissue was suspended in 1 mL of TRIzol and homogenized for 6 rounds of 10 seconds homogenization and 60-second holds between each round. Following homogenization, 300 µL of chloroform was added, and samples were centrifuged with heavy gel phase-lock tubes (VWR, 10847-802) to separate the aqueous phase.Library Construction - Library preparation (mRNA library, poly A enrichment) was performed by Novogene.Nucleic Acid Extraction - RNA purification kit (Quantabio, Extracta Plus, Cat# 95214-050) was used following the manufacturer’s protocol. RNA concentration and integrity were assessed with a Qubit 4.0 Fluorometer and Agilent Bioanalyzer prior to sequencing.Sequencing - RNA sequencing (NovoSeq PE150. 6G raw data per sample) was performed by Novogene.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMax ThorwaldfalseTranscriptomic responses to diesel exhaust particles or World trade center dustC57BL/6 Mouse cortex samples at 2-months of age, both sexes, exposed to one acute 5-hour dose of 100ug/m3 of air pollution from diesel exhaust particles or dust from the World Trade Center collapse. C57BL/6 female mouse cortex exposed to one acute 4-hour dose of 500ug/m3 of woodsmoke.2025-12-22T00:00:00Z2025-12-22T02:01:18.926Z2025-11-19T13:32:19.781ZE-MTAB-16199ERP185330EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184