biostudies-arrayexpressKohei KumegawaHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16203CUT&Tag for H3K27Ac; Colorectal cancer patient derived organoids, HCT26-1T; drug experiment of control vs GSK126.biostudies-arrayexpressNucleic Acid Extraction - CUT&Tag was performed using the CUT&Tag-IT Assay Kit (Active Motif, Cat. No. 53160) according to the manufacturer’s protocol.Sample Collection - Organoids cultured with GSK126 treatment or vehicle control were used as input material, with 2 × 10^5 cells per reaction.Library Construction - CUT&Tag was performed using the CUT&Tag-IT Assay Kit (Active Motif, Cat. No. 53160) according to the manufacturer’s protocol.Sequencing - Libraries were generated following the kit protocol and sequenced on a NextSeq 550 platform using paired-end reads.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Raw paired-end sequencing reads were processed to remove adapter sequences using Skewer (v0.2.2) with the NexteraPE adapter reference. Read quality was assessed with FastQC (v0.11.9). Adapter-trimmed reads were aligned to the human reference genome (hg38) using Bowtie2 (v2.3.5.1) with the parameters \"-- local --very-sensitive --no-mixed --no-discordant --phred33 -I 10 -X 700\". Only uniquely mapped reads were retained by excluding alignments containing the “XS” tag. Properly paired reads were extracted and converted to BAM format using SAMtools (v1.10). Peak calling was performed with MACS2 (v2.2.7.1) with the parameters “-g hs -f BAMPE --call-summits --keep-dup auto -q 0.01”.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsNextSeq 550CUT&RUNHomo sapiensKohei KumegawafalseCUT&Tag of H3K27Ac of HCT26-1T: Control and GSK126CUT&Tag for H3K27Ac; Colorectal cancer patient derived organoids, HCT26-1T; drug experiment of control vs GSK126.2025-11-20T00:00:00Z2026-05-27T17:48:20.062Z2025-11-19T15:42:44.787ZE-MTAB-16203ERP185340EFO_0002944EFO_0009973EFO_0004170EFO_0005518EFO_0003816EFO_0004184