biostudies-arrayexpressJustin OoiHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16204HMGB1 were silence in A549 cells using siRNA targeting.These A549 cells were then infected with WT Dengue virus 2 and its attenuated mutants (generated using infectious clone construction with site-directed mutagenesis) to better understand the gene expression changes induced by these different attenuated viruses and the role HMGB1 plays in this context.biostudies-arrayexpressSample Treatment - A549 cells were transfected with either siRNA targeting HMGB1 or non-targeting control siRNA twice at 24 hr interval using Lipofectamine RNAiMax reagentScaning - Hybridized samples were loaded on the nCounter cartridge and post-hybridization steps and scanning was performed on the nCounter Sprint Profiler. RCC files were analyzed using nSolver analysis software (Version 4.0) as per the manufacturer’s protocols.Nucleic Acid Extraction - Total RNA was extracted using the RNeasy Mini Kit as per manufacturer's instructions.Hybridization - Total RNA (50ng) was hybridized to reporter and capture probe sets at 65°C for 24h.Sample Collection - A549 cells were cultured in RPMI growth media supplemented with 10% fetal bovine serumLabeling - Not Applicable as this is a Nanostring platform and no labelling performedMIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsData Transformation - Negative and positive controls included in probe sets were used for background thresholding, and normalizing samples for differences in hybridization or sample input respectively.UnknownTranscriptomicsGenomicsProteomicstranscription profiling by arrayHomo sapiensReduced dengue virus pre-membrane protein-HMGB1 interaction activates host cell transcription to attenuate infection.Dengue virus pre-membrane protein bind HMGB1 to silence host transcriptional response for stealth during infectionSiriphanitchakorn T, Ooi JSG, Ng WC, Tan HC, Zhang SL, Ng DHL, Yap X, Yee JX, Zheng Q, Tan YT, Ong EZ, Chan KR, Choy MM, Ooi EE.Kuan Rong ChanTanamas Siriphanitchakorn, Justin S. G. Ooi, Wy Ching Ng, Hwee Cheng Tan, Summer L. Zhang, Dorothy H. L. Ng, Yap Xin, Jia Xin Yee, Zheng Qiao, Yi Ting Tan, Eugenia Z. Ong, Kuan Rong Chan, Milly M. Choy and Eng Eong OoiJustin OoifalseTranscriptional analysis (nCounter Human Immunology v2 Panel) of A549 cells with HMGB1 knockdown and infected with WT or attenuated mutant Dengue virus 2 strainsHMGB1 were silence in A549 cells using siRNA targeting.These A549 cells were then infected with WT Dengue virus 2 and its attenuated mutants (generated using infectious clone construction with site-directed mutagenesis) to better understand the gene expression changes induced by these different attenuated viruses and the role HMGB1 plays in this context.2026-05-14T00:00:00Z2026-05-14T18:44:28.849Z2025-11-19T15:20:08.265ZE-MTAB-16204publ-0-y2jg-removableEFO_0002768EFO_0002944EFO_0003814EFO_0003813EFO_0005518EFO_0003816EFO_0003815EFO_000396910.1038/s41541-026-01401-3