{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Santo Diprima"],"organism":["Mus musculus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16211"],"description":["Endothelial cells were isolated from the sciatic nerve and spinal cord of SOD1-G93A and non-transgenic littermate mice at two disease stages: onset (P90) and early symptomatic (P120). Following tissue dissociation, endothelial cells were purified by fluorescence-activated cell sorting. RNA was then extracted and subjected to bulk RNA sequencing to identify transcriptional signatures associated with disease progression."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - After quantification and quality control with Qubit 3.0 Fluorometer (Thermo Fisher Scientific) and Agilent 2100 Bioanalyzer, RNA-seq libraries were generated using the SMART-Seq Ultra Low Input RNA Kit (Illumina) (single-end, 100bp read length).","Sequencing - Sequencing was performed on an Illumina NovaSeq6000 system.","Sample Collection - For each replicate, 6-7 sciatic nerves and 3-4 spinal cords were pooled by genotype and dissociated. Control (non-transgenic) P90 sciatic nerves (3 independent replicates), SOD1G93A P90 sciatic nerves (3 replicates), Control P120 sciatic nerves (3 independent replicates), SOD1G93A P120 sciatic nerves (3 replicates), Control P90 spinal cords (3 replicates), SOD1G93A P90 spinal cords (3 replicates), Control P120 spinal cords (4 replicates), SOD1G93A P120 sciatic nerves (4 replicates). An average of 20,000 ECs were purified by FACS for each replicats after staining with rat anti-CD31 APC (1:50; BD Pharmingen Cat#551262) and rat anti-CD45.2 V405 (1:250; BD Biosciences Cat# 560697).","Nucleic Acid Extraction - ECs (CD31+/CD45−) were sorted with BD FACSAria Fusion flow cytometer into 400l of Trizol-LS (Thermo Fisher Scientific Cat # 10296010)."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Gene expression values were normalized using DESeq2’s median-of-ratios method for differential expression analysis"],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"species":["Mus musculus musculus"],"pubmed_authors":["Dario Bonanomi","Santo Diprima"],"additional_accession":[]},"is_claimable":false,"name":"Bulk RNA-seq of endothelial cells isolated from sciatic nerve and spinal cord of SOD1-G93 and control mice","description":"Endothelial cells were isolated from the sciatic nerve and spinal cord of SOD1-G93A and non-transgenic littermate mice at two disease stages: onset (P90) and early symptomatic (P120). Following tissue dissociation, endothelial cells were purified by fluorescence-activated cell sorting. RNA was then extracted and subjected to bulk RNA sequencing to identify transcriptional signatures associated with disease progression.","dates":{"release":"2025-12-11T00:00:00Z","modification":"2025-12-11T02:01:56.967Z","creation":"2025-11-20T00:59:02.178Z"},"accession":"E-MTAB-16211","cross_references":{"ENA":["ERP185356"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184"]}}