biostudies-arrayexpressAntonios PapadakisMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16217The goal of this study is to investigate the biological role of the podocyte-specific long non-coding RNA Wt1os (human ortholog WT1-AS) and its contribution to glomerular health and disease. We generated a Wt1os loss-of-function mouse model and assessed kidney morphology and function using histological techniques and high-resolution microscopy. Glomerular transcriptomes were profiled by total RNA sequencing. This dataset contains total RNA-Seq data from 3 wild type and 3 Wt1os loss-of-function mutant mice.biostudies-arrayexpressNucleic Acid Extraction - Total RNA was isolated from pelleted glomeruli using the Direct-zol RNA Kit (Zymo Research, USA) according to the manufacturer’s protocol. RNA concentrations were measured with the Qubit™ RNA Broad Range assay kit (Invitrogen), and RNA integrity was validated using the Tape Station system (Agilent).Library Construction - Sequencing libraries were prepared with the Illumina Stranded TruSeq Ribo Zero protocolSample Collection - Mice were sacrificed by cervical dislocation. Both kidneys were collected with the abdominal aorta attached. Kidneys were perfused through the renal arteries with magnetic beads (Dynabeads M-450 Tosylactivated, Thermo Fisher Scientific) in HBSS. Perfused kidneys were minced and incubated in digesting solution (1 mg/mL collagenase type II, 0.75 mg/mL pronase E, and 100 U/mL DNase I in HBSS) for 15 minutes at 37°C. Digested tissue was filtered through a 100-μm strainer twice. Glomeruli were isolated using magnetic separation, washed twice in HBSS, pelleted, and stored at -80°C.Sequencing - Paired end sequencing on Illumina NovaSeq 6000, 100 bpOrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Raw sequencing reads were processed using the nf-core/rnaseq pipeline version 3.13.2 (doi:10.5281/zenodo.1400710) with Nextflow version 23.04.1.5866, executed within a Singularity container. Quality and adapter trimming were performed using Trim Galore with default parameters. Transcript-level quantification was performed using Salmon with the following parameters: --numBootstraps 100, --validateMappings, --gcBias, and --seqBias. These parameters were applied to improve quantification accuracy and reduce potential biases related to sequence composition and fragment GC content.UnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of total RNAMus musculusAntonios PapadakisfalseWt1os lncRNA as a Novel Regulator of Podocyte Function (RNA-Seq of mouse glomeruli)The goal of this study is to investigate the biological role of the podocyte-specific long non-coding RNA Wt1os (human ortholog WT1-AS) and its contribution to glomerular health and disease. We generated a Wt1os loss-of-function mouse model and assessed kidney morphology and function using histological techniques and high-resolution microscopy. Glomerular transcriptomes were profiled by total RNA sequencing. This dataset contains total RNA-Seq data from 3 wild type and 3 Wt1os loss-of-function mutant mice.2026-06-01T00:00:00Z2026-06-01T01:00:56.262Z2025-11-20T02:14:48.891ZE-MTAB-16217ERP185359EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0003816EFO_0004184